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FITC anti-mouse Lineage Cocktail with Isotype Ctrl
FITC anti-mouse Lineage Cocktail with Isotype Ctrl
133301 25 tests $325.00     
133302 100 tests $550.00     
Product Details
Clone: 145-2C11, RB6-8C5, M1/70, RA3-6B2,Ter-119
Isotype: Armenian hamster IgG, Rat IgG2b, Rat IgG2a
Reactivity: Mouse
Formulation: This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation: The antibodies are purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution are free of unconjugated FITC.
Storage & Handling: This product is guaranteed for nine months. The antibody solutions should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested  
Recommended Usage:

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References:

1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
C57BL/6 bone marrow cells stained
C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 Alexa Fluor® 647

C57BL/6 bone marrow cells stained
C57BL/6 bone marrow cells stained with FITC isotype control cocktail and CD34 Alexa Fluor® 647


Compare all formats



Description:

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

 

Materials provided:

  1. FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 tests
  2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 tests

Materials not provided:

Cell Staining Buffer (Cat. No. 420201)
Other Names: lin-1
GeneID: 104231
Latest Publications: View the latest Lineage articles on HighwirePress.com
UniProt: View information about Lineage on UniProt.org
Keywords: FITC anti-mouse Lineage Cocktail with Isotype Ctrl, 145-2C11, RB6-8C5, M1/70, RA3-6B2,Ter-119, FITC, lin-1, Mouse, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • FITC anti-mouse Lineage Cocktail with Isotype Ctrl
    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with FITC Lineage Cocktail and CD34 Alexa Fluor® 647

    C57BL/6 bone marrow cells stained

    C57BL/6 bone marrow cells stained with FITC isotype control cocktail and CD34 Alexa Fluor® 647

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