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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 564101) as the standard. Flow Cytometry2-4,7,8,11,12: The fluorochrome-labeled TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17 -producing cells within mixed cell populations. View intracellular cytokine staining protocol Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906). Additional reported applications (for the relevant formats) include: Western blotting.
Application References:
1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611. 2. Schubert D, et al. 2004. J. Immunol. 172:4503. (FC) 3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (FC, ELISA Capture) 4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (FC, ELISA Capture) 5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture) 6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut) 7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (FC) 8. Kang SG, et al. 2007. J. Immunol. 179:3724. (FC) 9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed 10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed 11. Wang C, et al. 2009. Mucosal Immunol 2:173. (FC) PubMed 12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed 13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed 14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed 15. Nian H, et al. 2012. Invest Ophthalmol Vis Sci. 53:897. PubMed.
IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.
Other Names:
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Structure:
Cytokine; dimer; 15 kD (Mammalian)
Cellular Sources:
CD4+ memory T cells
Cellular Targets:
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors:
IL-17R (CD217)
Bioactivity/Activities:
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Antigen References:
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Numasaki M, et al. 2002. Blood 101:2620. 3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593. 4. Yao Z, et al. 1997. Cytokine 9:794. 5. Dong C. 2006. Nat. Rev. Immunol. 6:329. 6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse IL-17A
PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE
6 hours PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated mouse thymoma cell line EL-4 (in the presence of monensin) were intracellularly stained with IL-17 (clone TC11-18H10.1) Alexa Fluor® 700 (filled histogram) or rat IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Th17-polarized C57BL/6 mouse CD4+ lymphocytes were stimulated with PMA + Ionomycin for 6 hours in the presence of monensin, stained with CD4 FITC, fixed, permeabilized, and then stained with IL-17A (clone TC11-18H10.1) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 650™ anti-mouse IL-17A
Th17-polarized C57BL/6 mouse CD4+ lymphocytes were stimulated with PMA + Ionomycin for 6 hours (in the presence of monensin), stained with CD4 FITC, fixed, permeabilized, and then stained with IL-17A (clone TC11-18H10.1) Brilliant Violet 650™.
