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FITC anti-mouse IgD Antibody
FITC anti-mouse IgD Antibody
405703 50 µg $80.00     
405704 500 µg $210.00     
Product Details
Clone: 11-26c.2a
Isotype: Rat IgG2a, κ
Isotype Control:FITC Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3 of acetone-fixed frozen sections.
Application
References:

1. Nitschke, L., et al. 1993. P. Natl. Acad. Sci. 90:1887. (FC)
2. Weih, D., et al. 2001. J. Immunol. 167:1909. (IHC)
3. Koni, P.A., et al. 2001. J. Exp. Med. 193:741. (IHC)
4. Ahuja, A., et al.. 2007. J. Immunol. 179:3351. (FC) PubMed
5. Haynes, N.M., et al. 2007. J. Immunol. 179:5099. (FC)
6. Good-Jacobson, K.L., et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
7. Tomyako, M.M., et al. 2010. J. Immunol. 185:7146. PubMed
8. Sweet, RA., et al. 2011. Proc Natl Acad Sci U S A. 108:7932. PubMed.

C57BL/6 mouse splenocytes stained with
C57BL/6 mouse splenocytes stained with 11-26c.2a FITC


Compare all formats



Description:

Surface IgD is an important B cell differentiation marker.
Structure: Ig family
Distribution: B cells
Function: B cell differentiation
GeneID: 380797
Latest Publications: View the latest IgD articles on HighwirePress.com
UniProt: View information about IgD on UniProt.org
Keywords: FITC anti-mouse IgD, 11-26c.2a, FITC, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • FITC anti-mouse IgD
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 11-26c.2a FITC

  • PE anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a PE

    C57BL/6 splenocytes stained with 11-26c.2a PE

  • Purified anti-mouse IgD
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 11-26c.2a, followed by anti-rat IgG FITC

  • Alexa Fluor® 647 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647

  • PerCP/Cy5.5 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a PerCP/Cy5.5

  • Pacific Blue™ anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a Pacific Blue™ and CD19 antibody (6D5) PE

    C57BL/6 splenocytes stained with rat

    C57BL/6 splenocytes stained with rat IgG2a Pacific Blue™ isotype and CD19 antibody (6D5) PE

  • APC anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a APC

  • APC/Cy7 anti-mouse IgD
    C57BL/6 splenocytes stained with 11-26c.2a

    C57BL/6 splenocytes stained with 11-26c.2a APC/Cy7

  • Alexa Fluor® 488 anti-mouse IgD
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with B220 APC and IgD (11-26c.2a) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).





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