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FITC anti-mouse/human Helios Antibody
FITC anti-mouse/human Helios Antibody
137204 25 tests $140.00     
137214 100 tests $315.00     
Product Details
Clone: 22F6
Isotype: Armenian Hamster IgG
Isotype Control:FITC Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse, Human
Immunogen: Helios peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
References:

1. Thornton AM, et al. 2010. J. Immunol.. 184:1. PubMed
2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
3. Long, SA., et al. 2011. J. Immunol. 187:3511. PubMed.
4. Nakagome K, et al. 2011. J. Immunol. 187:5077. PubMed.

C57BL/6 splenocytes surface stained with
C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-FITC (clone 22F6).

Human peripheral blood lymphocytes surface
Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-FITC (clone 22F6).


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Description:

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is involved in tumor immunity.
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Antigen
References:

1. Kelly CM, et al.  1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.
GeneID: 22779
22807
Latest Publications: View the latest Helios articles on HighwirePress.com
UniProt: View information about Helios on UniProt.org
Keywords: FITC anti-mouse/human Helios, 22F6, FITC, Mouse, Human, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • FITC anti-mouse/human Helios
    C57BL/6 splenocytes surface stained with

    C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-FITC (clone 22F6).

    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-FITC (clone 22F6).

  • PE anti-mouse/human Helios
    C57BL/6 splenocytes surface stained with

    C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-PE (clone 22F6).

    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-PE (clone 22F6).

  • Alexa Fluor® 647 anti-mouse/human Helios
    C57BL/6 splenocytes surface stained

    C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Alexa Fluor® 647 (clone 22F6).

    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-PE (clone RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Alexa Fluor® 647 (clone 22F6).

  • Pacific Blue™ anti-mouse/human Helios
    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-PE (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Pacific Blue™ 647 (clone 22F6).

    C57BL/6 splenocytes surface stained with

    C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Pacific Blue™ (clone 22F6).

  • Alexa Fluor® 488 anti-mouse/human Helios
    C57BL/6 splenocytes surface stained

    C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-Alexa Fluor® 488 (clone 22F6).

    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-APC (clone RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-Alexa Fluor® 488 (clone 22F6).

  • APC anti-mouse/human Helios
    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-PE/Cy7, fixed and permeabilized with BioLegend FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-APC (clone 22F6).

    C57BL/6 mouse splenocytes surface stained

    C57BL/6 mouse splenocytes surface stained with CD4-PE/Cy7, fixed and permeabilized with BioLegend FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-APC (clone 22F6).

  • Purified anti-mouse/human Helios
    C57BL/6 splenocytes surface stained with

    C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-PE (clone 22F6).

    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD4-APC (RPA-T4), fixed and permeabilized using BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellulary stained with Helios-PE (clone 22F6).

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