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FITC anti-mouse CD8b Antibody
FITC anti-mouse CD8b Antibody
126605 50 µg $50.00     
126606 500 µg $200.00     
Product Details
Clone: YTS156.7.7
Isotype: Rat IgG2b, κ
Isotype Control:FITC Rat IgG2b, κ Isotype Ctrl
Reactivity: mouse CD8 beta chain
Immunogen: mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Application
References:

1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
BALB/c mouse splenocytes stained with
BALB/c mouse splenocytes stained with YTS156.7.7 FITC


Compare all formats



Description:

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
Other Names: Lyt-3, Ly-3
Structure: Ig superfamily, CD8β chain, 34 kD
Distribution: majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand Receptor: MHC class I
Antigen
References:

1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press.
2. Zamoyska R. 1994. Immunity 1:243-246.
3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523.
4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
GeneID: 12526
Latest Publications: View the latest CD8b articles on HighwirePress.com
UniProt: View information about CD8b on UniProt.org
Keywords: FITC anti-mouse CD8b, YTS156.7.7, FITC, Lyt-3, Ly-3, mouse CD8 beta chain, Immunology, Antibodies
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Cell Staining BufferFC, ICC, ICFC
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TruStain fcX™ (anti-mouse CD16/32)93 FC
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse CD8b
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC

  • Biotin anti-mouse CD8b
    BALB/c mouse splenocytes stained with

    BALB/c mouse splenocytes stained with biotinylated YTS156.7.7, followed by Sav-PE

  • FITC anti-mouse CD8b
    BALB/c mouse splenocytes stained with

    BALB/c mouse splenocytes stained with YTS156.7.7 FITC

  • PE anti-mouse CD8b
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YTS156.7.7 PE

  • PerCP/Cy5.5 anti-mouse CD8b
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with YTS156 PerCP/Cy5.5

  • Alexa Fluor® 647 anti-mouse CD8b
    57BL/6 mouse splenocytes stained with

    57BL/6 mouse splenocytes stained with YTS156.7.7 Alexa Fluor® 647

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