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FITC anti-mouse CD8a Antibody
Cat. # Size Price  

100803 50 µg $80.00
    
Reviews (3)
100804 500 µg $250.00
    
Reviews (3)
Product Details

Clone: 5H10-1 (See other available formats)
Isotype: Rat (SD) IgG2b, λ
Reactivity: Mouse
Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:
Blue Laser (488 nm)
Application
Notes:
Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2 of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.
Application
References:
  Publication Library
 Image 1
C57BL/6 mouse splenocytes stained with 5H10-1 FITC


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See FITC spectral data





 


Antigen Details

Description: CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Other Names: T8, Lyt2, Ly-2
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand Receptor: MHC class I molecule
Antigen
References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
GeneID: 12525
UniProt: View information about CD8a on UniProt.org
Keywords: FITC anti-mouse CD8a, 5H10-1, FITC, T8, Lyt2, Ly-2, Mouse, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
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Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD8a
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 5H10-1 FITC

  • Purified anti-mouse CD8a
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC

    Mouse OT-I primary CTL cells

    Mouse OT-I primary CTL cells were stained with purified anti-mouse CD8a primary antibody at 1:50 dilution in PBS/1%NGS incubated O/N at 4°C, detected with anti-rat IgG2b FITC at 1:2000 dilution for 1hr at room temperature (green), plus DAPI staining for nuclei (blue). Imaged with a Zeiss 200M deconvolution microscope (deconvolution applied). Negative control was anti-rat IgG2b isotype control plus secondary antibody, same conditions. Images courtesy of Dr. Javier Casas at The Scripps Research Institute.

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