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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2 of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.
Application References:
1. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. 2. Frei K, et al. 1997. J. Exp. Med. 185:2177.
C57BL/6 mouse splenocytes stained with 5H10-1 FITC
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Other Names:
T8, Lyt2, Ly-2
Structure:
Ig superfamily, CD8α chain, 34 kD
Distribution:
Most thymocytes, T cell subset, some NK cells
Function:
Co-receptor for TCR
Ligand Receptor:
MHC class I molecule
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD8a
C57BL/6 mouse splenocytes stained with 5H10-1 FITC
Purified anti-mouse CD8a
C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC
