Antibodies
|
Search   
FITC anti-mouse CD8a Antibody
FITC anti-mouse CD8a Antibody
100803 50 µg $80.00 Qty:
    
100804 500 µg $250.00 Qty:
    

Product Details

 
Clone: 5H10-1 (See other available formats)
Isotype: Rat (SD) IgG2b, λ
Reactivity: Mouse
Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2 of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.

Application
References:

  Publication Library
FITC anti-mouse CD8a Antibody 5H10-1 Image 1
C57BL/6 mouse splenocytes stained with 5H10-1 FITC


Compare all formats

See FITC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Other Names: T8, Lyt2, Ly-2
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand Receptor: MHC class I molecule
Antigen
References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

GeneID: 12525
Latest Publications: View the latest CD8a articles on HighwirePress.com
UniProt: View information about CD8a on UniProt.org
Keywords: FITC anti-mouse CD8a, 5H10-1, FITC, T8, Lyt2, Ly-2, Mouse, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
BioLegend 100804 (FC) /4 2012-11-18
BioLegend 100803 (FC) /4 2012-11-18
Review this product
See more reviews using the Reviews Library


Product FAQs

 
There are no FAQs for this product. For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
FITC anti-mouse CD3ε145-2C11FC
FITC anti-mouse CD317A2FC
FITC anti-mouse CD8a53-6.7FC
FITC anti-mouse CD4GK1.5FC
FITC anti-mouse CD4RM4-4FC
FITC anti-mouse CD4RM4-5FC
Cell Staining BufferFC, ICC, ICFC
TruStain fcX™ (anti-mouse CD16/32)93 FC


Other Formats

DescriptionCloneApplications
Purified anti-mouse CD8a5H10-1FC, IF, IHC


Related Pathways

Pathway
Dendritic Cells
Gamma-Delta T cells


Related Categories

Category
Immunology > Mouse Immunology > NK/NKT Cells > CD8a (Ly-2) > 5H10-1
Immunology > Mouse Immunology > CD and Related Molecules > CD8a > 5H10-1
Immunology > Mouse Immunology > Dendritic cells > CD8a > 5H10-1
Immunology > Mouse Immunology > T Regulatory Cells (TReg) > CD8
Immunology > Mouse Immunology > Stem Cells > CD8a

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD8a
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 5H10-1 FITC

  • Purified anti-mouse CD8a
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 5H10-1, followed by anti-rat IgGs-FITC

    Mouse OT-I primary CTL cells

    Mouse OT-I primary CTL cells were stained with purified anti-mouse CD8a primary antibody at 1:50 dilution in PBS/1%NGS incubated O/N at 4°C, detected with anti-rat IgG2b FITC at 1:2000 dilution for 1hr at room temperature (green), plus DAPI staining for nuclei (blue). Imaged with a Zeiss 200M deconvolution microscope (deconvolution applied). Negative control was anti-rat IgG2b isotype control plus secondary antibody, same conditions. Images courtesy of Dr. Javier Casas at The Scripps Research Institute.

x
Copyright © 2014 BioLegend, Inc. All rights reserved.