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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105108).
Application References:
1. Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060. 2. Nuriya S, et al. 1996. Int. Immunol. 8:917. 3. Kasprowicz DJ, et al. 2000. J. Immunol. 165:680. 4. Saito K, et al. 1998. J. Immunol. 160:4225. 5. Nakajima A, et al. 1998. J. Immunol. 161:1901. 6. Ma XT, et al. 2006. Cancer Research 66:1169. 7. Lawson BR, et al. 2007. J. Immunol. 178:5366. 8. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. 9. Giroux M, et al. 2007. J. Immunol. 179:4492.
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with PO3 FITC
CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.
Other Names:
B7-2, B70, Ly-58
Structure:
Ig superfamily, 80 kD
Distribution:
B and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function:
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD86
LPS-stimulated (3 days) BALB/c
splenocytes stained with PO3 PE
Purified anti-mouse CD86
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with purified PO3, followed by anti-rat IgG FITC
FITC anti-mouse CD86
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with PO3 FITC
APC anti-mouse CD86
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with PO3 APC
