The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested WB - This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Puls K.L. et al. 2002. Int. Immunol. 14(3):249
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
Description:
CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.
Other Names:
Structure:
A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
Distribution:
Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
Function:
Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
Interaction:
Interact with CD2, CD9, CD19, and CD21.
Antigen References:
1. Camo A.M. et al. 1995. Eur J Immunol. 25(7):2090 2. Tomlinson, M.G. et al. 1995. Eur J Immunol. 25(8):2201 3. Angelisova, P. et al. 1994. Immunogenetics. 39(4):249 4. Wright, M.D. et al. 1993. Int. Immunol. 5(2):209 5. Rasmussen, A. et al. 1994. J. Immunol. 153:4997
