Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Puls KL, et al. 2002. Int. Immunol. 14(3):249.
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.
Structure:
A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
Distribution:
Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
Function:
Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
Interaction:
Interact with CD2, CD9, CD19, and CD21.
Antigen References:
1. Camo AM, et al. 1995. Eur. J. Immunol. 25(7):2090 2. Tomlinson MG, et al. 1995. Eur. J. Immunol. 25(8):2201 3. Angelisova P, et al. 1994. Immunogenetics. 39(4):249 4. Wright MD, et al. 1993. Int. Immunol. 5(2):209 5. Rasmussen A, et al. 1994. J. Immunol. 153:4997.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD53
C57BL/6 splenocytes stained with purified OX-79 conjugated to FITC and CD4 (GK1.5) PE
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
FITC anti-mouse CD53
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
