The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Puls K.L. et al. 2002. Int. Immunol. 14(3):249
C57BL/6 splenocytes stained with OX-79 FITC and CD4 (GK1.5) PE
C57BL/6 splenocytes stained with rat IgM FITC isotype control and CD4 (GK1.5) PE
Description:
CD53 is a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). CD53 is highly conserved in evolution, mouse CD53 was 91% identical to rat and 82% identical to human CD53. Mouse CD53 has a molecular mass of 35-45 kD and expresses on virtually all peripheral leukocytes, immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets. CD53 is proposed to play an important role in thymopoiesis and leukocyte signal transduction. It was reported that CD53 interact with a number of proteins, including CD2, CD9, CD19, and CD21. Cross-linking of CD53 promotes B cell activation. It was found that there is a strong correlation between CD53 expression and positive selection of thymocytes.
Other Names:
Structure:
A 35-42 kD member of the tetraspanin/transmambrane-4 superfamily.
Distribution:
Expressed on peripheral leukocytes, some immature CD4-CD8- double negative thymocytes, and functionally mature CD4+CD8- or CD4-CD8+ single positive subsets.
Function:
Play an important role in leukocyte signal transduction, thymopoiesis, and positive selection of thymocytes.
Interaction:
Interact with CD2, CD9, CD19, and CD21.
Antigen References:
1. Camo A.M. et al. 1995. Eur J Immunol. 25(7):2090 2. Tomlinson, M.G. et al. 1995. Eur J Immunol. 25(8):2201 3. Angelisova, P. et al. 1994. Immunogenetics. 39(4):249 4. Wright, M.D. et al. 1993. Int. Immunol. 5(2):209 5. Rasmussen, A. et al. 1994. J. Immunol. 153:4997
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