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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry5 of frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 103610).
Application References:
1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block) 2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block) 3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) 4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block) 5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC) 6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10(MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4.
Other Names:
α4 integrin, VLA-4 α chain, integrin α4
Structure:
Integrin family, associates with integrin β1 or β7, 150 kD
Distribution:
T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function:
T cell costimulation, cell-cell and cell-matrix adhesion
Ligand Receptor:
VCAM-1, MAdCAM-1, fibronectin
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722. 3. Berlin C, et al. 1993. Cell 74:185. 4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD49d
C57BL/6 mouse splenocytes stained with biotinylated R1-2, followed by Sav-PE
FITC anti-mouse CD49d
C57BL/6 splenocytes stained with R1-2 FITC
LEAF™ Purified anti-mouse CD49d
C57BL/6 mouse splenocytes stained with LEAF™ purified R1-2, followed by anti-rat IgG FITC
PE anti-mouse CD49d
C57BL/6 splenocytes stained with R1-2 PE
Purified anti-mouse CD49d
C57BL/6 mouse splenocytes stained with purified R1-2, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD49d
C57BL/6 mouse splenocytes stained with R1-2 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD49d
C57BL/6 mouse splenocytes stained with R1-2 Alexa Fluor® 647
