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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of cell adhesion1-3, and immunohistochemistry of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays.
Application References:
1. Miyake S, et al. 1994. Eur. J. Immunol. 24:2000. 2. Noto K, et al. 1995. Int. Immunol. 7:835. 3. Arase H, et al. 2001. J. Immunol. 167:1141.
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC
CD49b is a 150 kD glycoprotein, also known as α2 integrin, VLA-2 α chain, Integrin α2 chain, and HMα2. It is a member of the integrin family, expressed on NK cells, a subset of splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, and platelets. By associating with CD29 (integrin β1 subunit), CD49b forms the VLA-2 (integrin α2β1 ) complex. It plays a critical role in both adhesion and lymphocyte activation. The primary ligands for CD49b are collagen, laminin, and fibronectin. The HMα2 antibody has been shown to be useful for partially blocking CD49b mediated interactions with collagen. Additionally, this antibody blocks staining of splenic NK cells by the monoclonal antibody DX5.
Integrin family, associates with CD29 (β1 integrin), 150 kD
Distribution:
NK cells, subset splenic CD4+ T cells, NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, epithelial cells, platelets
Function:
Adhesion, lymphocyte activation
Ligand Receptor:
Collagen, laminin, fibronectin
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Sasaki K, et al. 2003. Int. Immunol. 15:701. 3. Inoue O, et al. 2003. J. Cell Biol. 160:769.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD49b
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC
LEAF™ Purified anti-mouse CD49b
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC
PE anti-mouse CD49b
C57BL/6 splenocytes stained with HMα2 PE and 145-2C11 FITC
Purified anti-mouse CD49b
C57BL/6 mouse splenocytes stained with purified HMα2, followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse CD49b
C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 488 and DX5 PE
Alexa Fluor® 647 anti-mouse CD49b
C57BL/6 mouse splenocytes stained with HMα2 Alexa Fluor® 647 and DX5 PE
