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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.
Application References:
1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB) 2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB) 3. Onami TM, et al. 2002. J. Immunol. 168:6022. 4. van der Most RG, et al. 2003. Intl. Immunol. 15:119. 5. Chu VT, et al. 2007. J. Immunol. 179:5947. 6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed 7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It does not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.
Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation
Distribution:
Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.
Function:
Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
Ligand Receptor:
CD54, Siglec-1
Antigen References:
1. van den Berg TK, et al. 2001. J. Immunol. 166:3637. 2. Moore T, et al. 1994. J. Immunol. 153:4978. 3. Onami TM, et al. 2002. J. Immunol. 168:6022. 4. Tong J, et al. 2004. J. Exp. Med. 199:1277. 5. Jones AT, et al. 1994. J. Immunol. 153:3426.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
