The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.
Application References:
1. Farr A., et al., 1991. J. Histochem. Cytochem. 39:645. (FC, IHC) 2. Dooley J. et al., 2005. J. Immunol. 175:4331. (FC, IHC) 3. Hinterberger, M., et. al., 2010. Nat. Immunol. 11:512. (FC) PubMed 4. Gracz, AD., et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.
Other Names:
CD326, EGP40, MIC18, TROP1, KSA
Structure:
40 kd Single-pass type 1 glycoprotein with 293 amino acids, which consists of a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain and a 26 aa cytoplasmic domain. The extracellular domain contains two repeats of epiderma
Distribution:
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
