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FITC anti-mouse CD19 Antibody
FITC anti-mouse CD19 Antibody
101505 50 µg $75.00     
101506 500 µg $250.00     
Product Details
Clone: MB19-1
Isotype: Mouse IgA, κ
Reactivity: Mouse
Immunogen: mouse CD19+ pre-B cell line 300.19
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.
Application
References:

1. Sato S, et al. 1997. J. Immunol. 158:4662. (Costim)
2. Sato S, et al. 1997. P. Natl. Acad. Sci. USA 94:13158. (Costim)
3. Krop I, et al. 1996. Eur. J. Immunol. 26:238. (IP)
4. Sato S, et al. 1996. J. Immunol. 157:4371.
5. Stolk M, et al. 2006. J. Leukoc. Biol. doi:10.1189/jlb.1205739. (FC)
C57BL/6 mouse splenocytes stained with
C57BL/6 mouse splenocytes stained with MB19-1 FITC


Compare all formats



Description:

CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.
Other Names: B4
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B to mature B (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand Receptor: CD21, CD81, Leu-13
Antigen
References:

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF,et al. 1994. Immunol. Today 15:437.
GeneID: 12478
Latest Publications: View the latest CD19 articles on HighwirePress.com
UniProt: View information about CD19 on UniProt.org
Keywords: FITC anti-mouse CD19, MB19-1, FITC, B4, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse CD19
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified MB19-1, followed by anti-mouse IgA FITC

  • Biotin anti-mouse CD19
    C57BL/6 splenocytes stained with MB19-1

    C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE

  • FITC anti-mouse CD19
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with MB19-1 FITC

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