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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.
Application References:
1. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 2. Kouro T, et al. 2002. Blood 100:3672. 3. Maeda K, et al. 2005. Blood 106:879. 4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 mouse splenocytes stained with CD3 (145-2C11 APC) and SB/199 FITC
CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Other Names:
IL-7 receptor α chain, IL-7Rα
Structure:
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution:
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function:
T cell and immature B cell proliferation and development
Ligand Receptor:
IL-7
Antigen References:
1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125. 2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503. 3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610. 4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with purified SB/199, followed by biotinylated anti-rat IgG and Sav-PE
Biotin anti-mouse CD127 (IL-7Rα)
C57BL/6 splenocytes stained with SB/199 biotin followed by Sav-PE and CD3 (145-C11) FITC
FITC anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11 APC) and SB/199 FITC
Alexa Fluor® 488 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 APC and SB/199 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 PE and SB/199 Alexa Fluor® 647
PE anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 FITC and SB/199 PE
C57BL/6 mouse thymocytes stained with CD3 FITC and SB/199 PE
PerCP/Cy5.5 anti-mouse CD127 (IL-7Rα)
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and SB/199 PerCP/Cy5.5
APC anti-mouse CD127 (IL-7Rα)
C57BL/6 splenocytes stained with SB/199 APC and CD3 (145-2C11) FITC
