The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested IHC(Frozen)1,2*, IF3* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,2 of acetone-fixed frozen sections, and immunofluorescence microscopy3.
Application References:
1. Chen, J. W., et al., 1985. Arch. Biochem. Biophys. 239:574. 2. Chen, J. W., et al., 1986. Biochem. Soc. Symp. 51:97. 3. Rohrer, J., et al., 1996. J. Cell Biol. 132:565. 4. Steptoe, R.J., et al., 2007. J. Immunol. 178:2094.
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B FITC
Description:
CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. The mature CD107a is heavily glycosylated from a 40 kD core protein. It is expressed by macrophages, epithelial cells, endothelial cells, and some tumor cells. This molecule is located on the luminal side of lysosomes and has been suggested to play a role in the protection of lysosome membrane from lysosomal hydrolases. CD107a is a ligand for galaptin. Upon activation, CD107a is transferred to the surface of cell membrane. It plays a role in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression.
Other Names:
Lysosome-Associated Membrane Protein 1, LAMP-1, LGP-120
Structure:
Type I transmembrane glycoprotein, 40 kD core protein, mature protein is heavily glycosylated with 110-140 kD
Distribution:
Macrophages, epithelial cells, endothelial cells, some tumor cells, located on the luminal side of lysosomes or on the surface of cell membrane
Function:
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand Receptor:
Galaptin
Antigen References:
1. Barclay, A.N., et al. 1997. The Leucocyte Antigen Facts Book. 2nd edition. Academic Press. 2. Heffernan, M., et al. 1989. Cancer Res. 49: 6077. 3. Granger, B. L., et al. 1990. J. Bio. Chem. 265: 12036. 4. De Carvalho Bittencourt, M., et al. 2005. Eur. J. Immunol. 35: 1501.
