The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
C57BL/6 thymocytes stained with CW-1.2 FITC
Description:
CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response.The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.
Other Names:
GPR 9-6, CDw199, Chemokine Receptor 9
Structure:
A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
Distribution:
Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on gamma/delta T cells in the intraepithelial and small intesti
Ligand Receptor:
CCL25
Interaction:
CCL25
Antigen References:
1. Zaballos A et al. 1999. J Immunol 162:5671 2. Wurbel MA et al. 2007. J Immunol 178:7598 3. Wurbel MA et al. 2006. Eur J Immunol 36:73
