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The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
This clone has been published as 1H9.
Additional reported applications (for the relevant formats) include: Western blot1, immunohistochemical staining with paraffin-embedded tissues section1,2.
Application References:
1. Robinson MJ, et al. 2002. J. Biol. Chem. 277:3658. (WB, IHC) 2. Croce K, et al. 2009. Circulation 120:427. (IHC)
Human peripheral blood granulocytes surface stained with anti-human CD15-PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (middle)
MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and recently has been considered an endogenous activator of Toll-like receptor 4. A recent report has identified S100A9 as a marker for CD14(+) HLA-DR(-/low) MDSCs.
Belongs to S100 family of protein containing 2-EF-hand calcium –binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8
Distribution:
MDSCs, Neutrophil, monocyte, and ileal tissue eosinophils
Function:
Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction:
Calcium
Antigen References:
1. Loser K, et al. 2010. Nat. Med. 16:713. 2. Cheng P, et al. 2008. J. Exp. Med. 205:2235. 3. Leukert N, et al.2006. J. Mol. Biol. 359:961. 4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402. 5. Zhao F, et al. 2012. Immunology. 136:176.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human MRP-14 (S100A9)
Human peripheral blood granulocytes surface stained with anti-human CD15 PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (bottom)
FITC anti-human MRP-14 (S100A9)
Human peripheral blood granulocytes surface stained with anti-human CD15-PerCP/Cy5.5, fixed and permeabilized, then intracellularly stained with anti-human MRP14-FITC (clone 1H9) (top) or mouse IgG1 FITC isotype control (middle)
