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The antibodies are purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Human peripheral blood leukocytes stained with FITC anti-Human Lineage Cocktail (CD3, CD14, CD19, CD20, CD56) and HLA-DR PerCP
Human peripheral blood co-stained with CD11c APC and CD16 PE, gated on the lineage negative/dim population
This anti-Human Lineage Cocktail is optimized for the detection of human lymphocytes, monocytes, eosinophils, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells (DCs), including DCs that express CD16. This cocktail is composed of CD3, CD14, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.
Other Names:
Lin2
Distribution:
CD3 is expressed on all T lymphocytes. CD14 is expressed on monocytes, macrophages, neutrophils, and eosinophils. CD19 and CD20 are expressed on B lymphocytes. CD56 is expressed on activated and resting NK lymphocytes.
Antigen References:
1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. 2. Olweus J, et al. 1997. P. Natl. Acad. Sci. USA 94:12551. 3. Banchereau J, et al. Eds. 1995. Advances in Experimental Medicine and Biology. New York. 4. Markowicz S, et al. 1990. J. Clin. Invest. 85:955 5. Freudenthal P, et al. 1990. P. Natl. Acad. Sci. USA 87:7698. 6. Macdonald K, et al. 2002. Blood 100:4512. 7. Piccioli D, et al. 2007. Blood 109:5371.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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