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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
ELISA Detection: The biotinylated E8N1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified H8A5 (Cat. No. 511502) antibody as the capture antibody. Flow Cytometry: The fluorochrome-labeled E8N1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-8-producing cells within mixed cell populations. View intracellular cytokine staining protocol Note: For testing human IL-8 in serum or plasma, BioLegend's ELISA Max™ Sets are specially developed and recommended.
LPS stimulated (6hrs) and monensin treated human peripheral blood monocytes surface stained with HCD14 APC and intracellularly stained with E8N1 FITC
IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human IL-8
FITC anti-human IL-8
LPS stimulated (6hrs) and monensin treated human peripheral blood monocytes surface stained with HCD14 APC and intracellularly stained with E8N1 FITC
PE anti-human IL-8
LPS-stimulated (6 hours, in the presence of monensin) human peripheral blood monocytes intracellularly stained with E8N1 PE
Alexa Fluor® 488 anti-human IL-8
LPS-stimulated (6 hours, in the presence of monensin) human peripheral blood monocytes intracellularly stained with E8N1 Alexa Fluor® 488
