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FITC anti-human IL-1β Antibody
FITC anti-human IL-1β Antibody
511705 25 tests $80.00     

Product Details

Clone: H1b-98
Isotype: Mouse IgG1, κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl (ICFC)
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

COA:
Enter Lot#:   
LPS-stimulated peripheral blood mononuclear cells
LPS-stimulated peripheral blood mononuclear cells intracellularly stained with H1b-98 FITC (gated on monocyte population)


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Description:

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. 

Other Names: Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b)
Structure: Cytokine; 17.3 kD (Mammalian)
Regulation: Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Cellular Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Cellular Targets: B cells, T cells, monocytes
Receptors: Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Bioactivity/Activities: Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia
Antigen
References:

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C. 1996. Blood 87:2095.

GeneID: 3553
Latest Publications: View the latest IL-1beta articles on HighwirePress.com
UniProt: View information about IL-1beta on UniProt.org
Keywords: FITC anti-human IL-1β, H1b-98, FITC, Interleukin-1β, H1, OAF, Catabolin, IFNb-inducing factor(il-1b,il1b) , Human, Immunology, Antibodies
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Cell Staining BufferFC, ICC, ICFC
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human IL-1β
    LPS-stimulated peripheral blood mononuclear cells

    LPS-stimulated peripheral blood mononuclear cells intracellularly stained with H1b-98 FITC (gated on monocyte population)

  • Pacific Blue™ anti-human IL-1β
    LPS-stimulated (6 hours) human peripheral

    LPS-stimulated (6 hours) human peripheral blood monocytes stained with CD14 PE and intracellular stained with H1b-98 Pacific Blue™

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