Antibodies
Login
Search   
FITC anti-human IgM Antibody
FITC anti-human IgM Antibody
314506 100 tests $160.00     
Product Details
Clone: MHM-88
Isotype: Mouse IgG1, κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Immunogen: Human IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Enter Lot#:   
Application
Notes:

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.
Human peripheral blood lymphocytes stained
Human peripheral blood lymphocytes stained with MHM-88 FITC


Compare all formats



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
GeneID: 959
Latest Publications: View the latest IgM articles on HighwirePress.com
UniProt: View information about IgM on UniProt.org
Keywords: FITC anti-human IgM, MHM-88, FITC, Immunoglobulin M , Human, Immunology, Antibodies
Related Products
DescriptionCloneApplications
FITC Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-human IgM
    Over night cultured human peripheral

    Over night cultured human peripheral blood lymphocytes stained with purified MHM-88, followed by anti-mouse IgG FITC

  • PE anti-human IgM
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human IgM (clone MHM-88) PE (top) or mouse IgG1, κ PE isotype control (bottom).





  • Biotin anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD19 APC and biotinylated MHM-88, followed by Sav-PE

  • FITC anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHM-88 FITC

  • APC anti-human IgM
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MHM-88 APC

  • PerCP/Cy5.5 anti-human IgM
    Overnight cultured human peripheral blood

    Overnight cultured human peripheral blood lymphocytes stained with MHM-88 PerCP/Cy5.5

  • Pacific Blue™ anti-human IgM
    Over night cultured human peripheral

    Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom)





  • Brilliant Violet 421™ anti-human IgM
    Isolated human peripheral blood mononuclear

    Isolated human peripheral blood mononuclear cells were stained with CD19 FITC and IgM (clone MHM-88) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). Data shown was gated on lymphocyte population.





  • APC/Cy7 anti-human IgM
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were cultured overnight and then stained with CD19 PE and anti-human IgM (clone MHM-88) APC/Cy7 (top) or mouse IgG1, κ APC/Cy7 isotype control (bottom).





  • Brilliant Violet 570™ anti-human IgM
    Isolated human peripheral blood mononuclear

    Isolated human peripheral blood mononuclear cells were stained with CD19 FITC and anti-human IgM (clone MHM-88) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom). Data shown was gated on lymphocyte population.





x
Copyright © 2012 BioLegend, Inc. All rights reserved.