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The FOXP3 antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The FOXP3 antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.
Surface Staining & FOXP3 Buffer Preparation:
Centrifugation steps: perform at 250Xg for 5min Incubation steps: perform at room temperature
1. Perform cell surface staining if necessary (See protocol: Cell Surface Immunofluorescence Staining Protocol). 2. Prepare 1x buffer solutions of FOXP3 Fix/Perm buffer and FOXP3 Perm buffer in PBS.
NOTE: The FOXP3 Perm buffer (10X) may have crystalization or precipitation observed when it is stored at 2-8°C, however, it is normal and does not affect the buffer performance. If there is a heavy precipitation observed after diluting to 1X working solution, it may be clarified by filtering. Caution: The FOXP3 Fix/Perm buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contact.
FOXP3 Intracellular Staining Procedures:
3. Add 1 ml of 1x Biolegend's FOXP3 Fix/Perm solution to each tube, resuspend the cells (gentle vortex) and incubate in the dark for 20 minutes, then centrifuge and remove the supernatant. The cell pellet will now be translucent and difficult to see; take care not to dislodge and accidentally aspirate cells at all later stages of staining protocol. 4. Wash: resuspend cells in cell staining buffer (Cat. No. 420201); centrifuge, then discard the supernatant. 5. Wash: resuspend in 1ml 1X BioLegend's FOXP3 Perm buffer; centrifuge, then discard the supernatant. 6. Resuspend cells in 1ml 1X BioLegend's FOXP3 Perm buffer, incubate in the dark for 15 minutes; centrifuge, then discard the supernatant. Resuspend the pellet in 100 µl of 1X BioLegend's FOXP3 Perm buffer. 7. Add appropriate amount of flurochrome conjugated anti-FOXP3 antibody and incubate in the dark for 30 minutes. 8. Wash twice with cell staining buffer (see step 4) then resuspend in 0.5ml cell staining buffer. Analyze with flow cytometer using appropriate instrument settings.
NOTE: BioLegend's FOXP3 Fix/Perm buffer set (Cat. No. 421403) is specifically developed and formulated for intracellular staining FOXP3 with minimum effect on surface fluorochrome staining and is highly recommended for optimal result of FOXP3 intracellular immunofluorescence staining.
Application References:
1. Roncador G, et al. 2005 Eur. J. Immunol. 35:1681. 2. Yang ZZ, et al. 2006. Blood 107:3639. 3. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed 4. Bollyky PL, et al. 2007. J. Immunol. 179:744. 5. Bell MP, et al. 2007. J. Immunol. 179:1893. 7. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed 8. Gao Q,et al.2007.J Clin Oncol.25:2586.PubMed 9. Pillai V,et al. 2008. Blood 111:463.PubMed 10. Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed 11. Zonios DI, et al. 2008.Blood112:287. PubMed 12. Kavanagh B, et al. 2008. BloodPubMed 13. Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed 14. Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed 15. Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed 16. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed 17. Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed 18. Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
Human peripheral blood lymphocytes surface stained with PE/Cy5 CD4 (RPA-T4) and PE CD25 (BC96), then intracellularly stained with FITC 206D. The data shown was gated on CD4+ cells.
FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25- cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in "scurfy" mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2. The 206D antibody recognizes human FOXP3 epitope in the region of amino acids 105-235.
Other Names:
Forkhead box protein P3, Scurfin, JM2, IPEX, Zinc finger protein JM2
Structure:
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution:
Nuclear; expressed in T regulatory cells
Function:
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Regulation:
FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
Interaction:
Interacts with DNA
Antigen References:
1. Hori S, et al. 2003. Science 299:1057. 2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human FOXP3
Cell extract from HEK293T cells transfected with human FoxP3 cDNA was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-FoxP3 antibody (clone 206D). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Alexa Fluor® 488 anti-human FOXP3
Human PBMCs were surface stained with CD4-APC, CD25-PE and intracellular stained with 206D Alexa Fluor® 488
Alexa Fluor® 647 anti-human FOXP3
Human peripheral blood lymphocytes surface stained with FITC CD4 (RPA-T4) and then intracellularly stained with Alexa Fluor® 647 206D
FITC anti-human FOXP3
Human peripheral blood lymphocytes surface stained with PE/Cy5 CD4 (RPA-T4) and PE CD25 (BC96), then intracellularly stained with FITC 206D. The data shown was gated on CD4+ cells.
Pacific Blue™ anti-human FOXP3
Dot plots of human peripheral blood lymphocytes stained with CD4-PE/Cy7, CD25-PE, and FOXP3 206D-Pacific Blue™.
PE anti-human FOXP3 Flow Kit
Human peripheral blood lymphocytes surface stained with CD4 FITC and then intracellular stained with 206D PE by using PE anti-human FOXP3 Flow Kit. Quadrant markers were set based on the staining of PE mIgG1, κ isotype control.
Alexa Fluor® 488 anti-human FOXP3 Flow Kit
Human peripheral blood lymphocytes surface stained with CD4 APC and then intracellular stained with 206D Alexa Fluor® 488 by using Alexa Fluor® 488 anti-human FOXP3 flow kit. Quadrant markers were set based on the staining with Alexa Fluor® 488 mIgG1, κ isotype control
PE anti-human FOXP3
Human peripheral blood lymphocytes surface stained with CD4 FITC and then intracellular stained with 206D PE
Alexa Fluor® 647 anti-human FOXP3 Flow Kit
Human peripheral blood lymphocytes were surface stained with CD4 PerCP and then intracellularly stained with 206D Alexa Fluor® 647 by using Alexa Fluor® 647 anti-human FOXP3 Flow Kit. Quadrant markers were set based on the staining of mouse IgG1, κ Alexa Fluor® 647 isotype control.
