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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications include: western blot, immunoprecipitation
Application References:
1. Hamann J, et al. 1995. J. Immunol. 155:1942. 2. Lawrence DW, et al. 2003. J. Exp. Med. 198:999. 3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood granulocytes stained with VIM3b FITC
CD97 is a seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family with molecular weights of 74 kD, 80 kD, and 86 kD. It is expressed on monocytes/macrophages, granulocytes, dendritic cells, and smooth muscle cells.The expression of CD97 on resting T- and B-lymphocytes is at low level, but is rapidly upregulated upon activation. CD97 binds to CD55 (decay-accelerating factor), chondroitin sulfate, integrin a5b1, and a3b1. CD97 has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.
Other Names:
BL-KDD/F12
Structure:
Seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family, 74 kD, 80 kD, 86 kD
Distribution:
Monocytes/macrophages, granulocytes, dendritic cells, low levels on T- and B-lymphocytes and upregulated upon activation
1. Capasso M, et al. 2006. J. Immunol. 177:1070 2. Wang T, et al. 2005. Blood 105:2836 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD97
PHA-stimulated (day-3) human peripheral blood mononuclear cells stained with purified VIM3b, followed by anti-mouse IgG FITC
FITC anti-human CD97
Human peripheral blood granulocytes stained with VIM3b FITC
PE anti-human CD97
Human peripheral blood granulocytes stained with VIM3b PE
Alexa Fluor® 647 anti-human CD97
Human peripheral blood granulocytes stained with VIM3b Alexa Fluor® 647
