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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Human peripheral blood lymphocytes stained with 3F3 FITC
CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.
Other Names:
Lyb-2, Ly-19.2, Ly-32.2
Structure:
Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Distribution:
Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells
Function:
negative regulation of B cell receptor signaling
Ligand Receptor:
CD5, CD100/Sema4D
Antigen References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schwarting T, et al. 1992. Am. J. Hematol. 41:151. 3. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155. 4. Kumanogoh A, et al. 2000. Immunity 13:621. 5. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75. 6. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD72
Human peripheral blood lymphocytes stained with purified 3F3,followed by anti-mouse IgGs FITC
FITC anti-human CD72
Human peripheral blood lymphocytes stained with 3F3 FITC
