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The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: Western blotting1,2, immunohistochemistry2, and immunprecipitation1,2.
Application References:
1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB) 2. Anzai N, et al. 2002. Blood 99:4413. (IHC, IP, WB)
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-human CD63
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
FITC anti-human CD63
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
Pacific Blue™ anti-human CD63
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
APC anti-human CD63
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
PE/Cy7 anti-human CD63
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).
