The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Schlossman, S., et al., Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Human peripheral blood lymphocytes stained
with p282(H19) FITC
Description:
CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.
Other Names:
Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
Structure:
Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
Distribution:
All cell types
Function:
Blocks complement-mediated lysis by inhibiting MAC formation
Ligand Receptor:
Complement components C8, C9
Antigen References:
1. Davies, A., et al., 1993. Immunol. Res. 12:258. 2. Lachmann, P. 1991. Immunol. Today 12:312. 3. Liszewski, M., et al., 1996. Adv. Immunol. 61:201.
