The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Alonzo, H., et al. 1984. Proc. Natl. Acad. Sci. USA 81:6681 2. Cattoretti, G, et al. 1993. Blood. 81:1726 3. Kamke, M.R., et al. 2005. Hear Res. 206:89
Human neuroblastoma cell line SK-N-MC stained with ME20.4 FITC
Description:
CD271 also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, is a type I transmembrane protein with a MW of 75 kDa, expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands, (NGF, BDNF, NTF3 and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.
Type I transmembranal protein with a MW of 75 kDa. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution:
Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes
1. Friedman, M.J. et al. 2009. J Biol Chem. 284:27944 2. Rock, J.R. et al. 2009. Proc Natl Acad Sci U S A. 106:12771 3. Kidd, S.K. et al. 2008. Brain Res.1195:113 4. Peters, E.M., et al. 2007. Am J Pathol. 171:1872 5. Jansen, P. et al. 2007. Nat Neurosci. 10:1449
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