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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.
Application References:
1.Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 3. Patton KM, et al. 2005 Infect Immun.73:2083.PubMed 4. Curti A, et al. 2010. Haematologica. 95:2022. PubMed
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 FITC
CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.
Other Names:
T6, R4
Structure:
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 FITC
PE anti-human CD1a
Molt-4 (T leukemia cell line) stained
with HI149 PE
PE/Cy5 anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 PE/Cy5
APC anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 APC
Purified anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with purified HI149, followed by anti-mouse IgGs FITC
Biotin anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with biotinylated HI149, followed by Sav-PE
Alexa Fluor® 488 anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 Alexa Fluor® 647
Pacific Blue™ anti-human CD1a
Human T leukemia cell line, Molt-4, stained with HI149 Pacific Blue™
Alexa Fluor® 700 anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 Alexa Fluor® 700
Brilliant Violet 421™ anti-human CD1a
Human T leukemia cell line (MOLT-4) was stained with CD1a (clone HI149) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
PE/Cy7 anti-human CD1a
Human T lymphoblastic leukemia cell line Molt-4 stained with HI149 PE/Cy7
APC/Cy7 anti-human CD1a
Human T-lymphoblastic leukemia cell line Molt-4 were stained with CD1a (clone HI149) APC/Cy7 (filled histogram) or mouse IgG1, κ APC/Cy7 isotype control (open histogram).
