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The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.
COA:
Application Notes:
Additional reported applications (for relevant formats) include: immunoprecipitation1, 2. Note: the antibody TS2/4 recognizes the β-propeller domain of CD11a and only immunoprecipitates the CD11a/CD18 complex.
Application References:
1. Huang C and Springer TA. 1997. P. Natl. Acad. Sci. USA 94:3162. (IP) 2. Sanchez-Madrid F, et al. 1983. J. Exp. Med. 158:1785. (IP) 3. Sanchez-Madrid F, et al. 1982. P. Natl. Acad. Sci. USA 79:7489. (FC)
Human peripheral blood lymphocytes stained with FITC anti-human CD11a (clone TS2/4, filled histogram) or FITC mouse IgG1, κ isotype control (open histogram)
CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).
Other Names:
LFA-1α chain, Integrin αL subunit, αL Integrin
Structure:
Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution:
Leukocytes
Function:
Adhesion, costimulation
Ligand Receptor:
ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Antigen References:
1. Lub M, et al. 1995. Immunol. Today 16:479. 2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD11a
Human peripheral blood lymphocytes stained with PE anti-human CD11a (clone TS2/4, filled histogram) or PE mouse IgG1, κ isotype control (open histogram)
FITC anti-human CD11a
Human peripheral blood lymphocytes stained with FITC anti-human CD11a (clone TS2/4, filled histogram) or FITC mouse IgG1, κ isotype control (open histogram)
PE anti-human CD11a
Human peripheral blood lymphocytes stained with PE anti-human CD11a (clone TS2/4, filled histogram) or PE mouse IgG1, κ isotype control (open histogram)
PerCP anti-human CD11a
Human peripheral blood lymphocytes stained with PerCP anti-human CD11a (clone TS2/4, filled histogram) or PerCP mouse IgG1, κ isotype control (open histogram)
