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FITC anti-human CD11a Antibody
FITC anti-human CD11a Antibody
301206 100 tests $160.00 Qty:
    

Product Details

 
Clone: HI111 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl
Workshop
Number:
IV N231
Reactivity: Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301214).

Application
References:

  Publication Library
FITC anti-human CD11a Antibody HI111 Image 1
Human peripheral blood lymphocytes stained with HI111 FITC


Compare all formats

See FITC spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Other Names: LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
Structure: Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
Distribution: Leukocytes
Function: Adhesion, costimulation
Ligand Receptor: ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
Antigen
References:

1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.

GeneID: 3683
Latest Publications: View the latest CD11a articles on HighwirePress.com
UniProt: View information about CD11a on UniProt.org
Keywords: FITC anti-human CD11a, HI111, FITC, LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL, Human, Cross-Reactivity: Chimpanzee, Baboon, Capuchin Monkey, Horse (Equine), Cattle (Bovine, Cow), Sheep (Ovine), Dog (Canine), Rabbit (Lapine), Flow Cytometry, Immunology, Antibodies


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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human CD11aHI111FC
Alexa Fluor® 647 anti-human CD11aHI111FC
APC anti-human CD11aHI111FC
Biotin anti-human CD11aHI111FC
LEAF™ Purified anti-human CD11aHI111FC, Block, IHC, WB
PE anti-human CD11aHI111FC
PE/Cy5 anti-human CD11aHI111FC
PE/Cy7 anti-human CD11aHI111FC
Purified anti-human CD11aHI111FC, IHC, WB


Related Pathways

Pathway
TH1 Pathway (Cellular Immune Response)
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > Myeloid Derived Suppressor Cells (MDSCs) > CD11a
Immunology > Human Immunology > Innate Immunity > CD11a (integrin αL)
Immunology > Human Immunology > Stem Cells > CD11a (integrin αL)
Immunology > Human Immunology > T Regulatory Cells (TReg) > CD11a (integrin αL)
Immunology > Non-Human Primate Immunology > CD11a (Integrin &alphaL)
Immunology > Human Immunology > Th1/Th2 > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Th17 > CD11a (integrin αL) > HI111
Immunology > Human Immunology > CD and Related Molecules > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Adhesion Molecules > CD11a (integrin αL) > HI111
Immunology > Human Immunology > Costimulatory Molecules > CD11a (integrin αL) > HI111
Cell Biology > Cell Adhesion > Integrin αL/CD11a

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 2 Revision Date: 2013-02-14
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 APC

  • Biotin anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated HI111, followed by Sav-PE

  • FITC anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 FITC

  • LEAF™ Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with LEAF™ purified HI111, followed by anti-mouse IgGs FITC

  • PE anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE

  • PE/Cy5 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 PE/Cy5

  • Purified anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified HI111, followed by anti-mouse IgGs FITC

  • Alexa Fluor® 488 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human CD11a
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with HI111 Alexa Fluor® 647

  • PE/Cy7 anti-human CD11a
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained CD11a (clone HI111) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 isotype control (open histogram).

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