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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.
Application References:
1. Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. 2. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. 3. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) 4. Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) 5. Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF)
Balb/c splenocytes intracellularly stained with 6H2.B4 FITC
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
Other Names:
Cyt c
Structure:
Heme binding domain; 15 kD
Distribution:
Mitochondrial intermembrane space
Function:
Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9
1. Liu X, et al. 1996. Cell. 86:147. 2. Li P, et al. 1997. Cell. 91:479. 3. Zhang Z, et al. 2003. Gene 312:61. 4. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-Cytochrome c
Balb/c splenocytes intracellularly stained with 6H2.B4 FITC
Purified anti-Cytochrome c
Cytochrome c was immunoprecipitated from Hela cell extract (1% NP-40) using 2-4 µg 6H2.B4 antibody/1 x107 cell equivalents. Immunoprecipitates were resolved by electrophoresis, transferred to nitrocellulose, and probed with the 7H8.2C12 anti-cytochrome c antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. In addition to the specific 15 kD cytochrome c band immunoprecipitated by 6H2.B4, heavy and light immunoglobulin chains are recognized by the goat anti-mouse secondary antibody.
