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FITC anti-CD247 (TCRζ, CD3ζ) Antibody
Cat. # Size Price  

644103 25 µg $105.00
    
644104 100 µg $265.00
    
Product Details

Clone: 6B10.2 (See other available formats)
Isotype: Mouse IgG1 κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Human TCR zeta chain aa38-54
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:
Blue Laser (488 nm)
Application
Notes:
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC


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See FITC spectral data





 


Antigen Details

Description: The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.
Other Names: CD247, CD3zeta
Structure: 163 amino acid long protein with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR alpha/beta
Antigen
References:
1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709
GeneID: 919
UniProt: View information about CD247 on UniProt.org
Keywords: FITC anti-CD247 (TCRζ, CD3ζ), 6B10.2, FITC, CD247, CD3zeta , Human, Intracellular Staining for Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
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Intracellular Flow Cytometry Staining Protocol
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-CD247 (TCRζ, CD3ζ)
    Jurkat (1) and Raiji (2)

    Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

  • FITC anti-CD247 (TCRζ, CD3ζ)
    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC

  • PE anti-CD247 (TCRζ, CD3ζ)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).





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