Product Search  
FITC anti-CD247 (TCRζ, CD3ζ) Antibody
Cat. # Size Price  

644103 25 µg $105.00
Reviews (3)
644104 100 µg $265.00
Reviews (3)
Product Details

Clone: 6B10.2 (See other available formats)
Isotype: Mouse IgG1 κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Human TCR zeta chain aa38-54
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Blue Laser (488 nm)
This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

(PubMed link indicates BioLegend citation)
  Publication Library
RRID: AB_2244275 (BioLegend Cat. No. 644103)
AB_2072959 (BioLegend Cat. No. 644104)
 Image 1
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC

Compare all formats

See FITC spectral data


Antigen Details

Description: The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.
Other Names: CD247, CD3zeta
Structure: 163 amino acid long protein with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR alpha/beta
1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709
GeneID: 919
UniProt: View information about CD247 on
Keywords: FITC anti-CD247 (TCRζ, CD3ζ), 6B10.2, FITC, CD247, CD3zeta , Human, Intracellular Staining for Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Safety Data Sheet
Certificate of Analysis
Request Bulk Quote
Request Custom Conjugation
Email Technical Support
Write a Review
View All CD247 Reagents
Related Protocols

Intracellular Flow Cytometry Staining Protocol

   Average Rating
Submit Review
Related Pages

Quality Control
CD Molecules Mobile Application
CD Molecule Chart
Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-CD247 (TCRζ, CD3ζ)
    Jurkat (1) and Raiji (2)

    Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

  • FITC anti-CD247 (TCRζ, CD3ζ)
    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC

  • PE anti-CD247 (TCRζ, CD3ζ)
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with Fixation Buffer and the Permeabilization buffer. Cells were then stained with CD247 (clone 6B10.2) PE (top) or mouse IgG1, κ PE isotype control (bottom).

9727 Pacific Heights Blvd.
San Diego, CA 92121

Tel: 877.246.5343
Web Tools
Explore & Learn
Custom Products/Services
Custom Panel Construction
Technical Support
Support Resources
Connect with us:

Facebook Twitter Pinterest Instagram LinkedIn YouTube Tumblr Blog Podcast
The LEGENDeers Pandora Google+ Wikipedia RSS Feed Arcade Words With Elements Satisfaction Survey
Privacy Policy | Terms
World-Class Quality | Superior Customer Support | Outstanding Value Copyright © 2014 BioLegend, Inc. All rights reserved.
Product Search