FITC anti-CD247 (TCRζ, CD3ζ) Antibody
FITC anti-CD247 (TCRζ, CD3ζ) Antibody
644103 25 µg $105.00 Qty:
644104 100 µg $265.00 Qty:

Product Details

Clone: 6B10.2 (See other available formats)
Isotype: Mouse IgG1 κ
Isotype Control:FITC Mouse IgG1, κ Isotype Ctrl (FC)
Reactivity: Human
Immunogen: Human TCR ζ chain aa38-54
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Blue Laser (488 nm)
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  Publication Library
FITC anti-CD247 (TCRζ, CD3ζ) Antibody 6B10.2 Image 1
Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC

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See FITC spectral data

Intracellular Flow Cytometry Staining Protocol


The invariant TCR ζ chain is a member of the CD3 complex associated with the clonotypic α/β TCR hetrodimer. The disulfide-linked TCRζ homodimers transmit signals following TCR ligation. Loss of TCR ζ expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.

Other Names: CD247, CD3ζ
Structure: 163 amino acid long protein with a short of extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR α/β

1. Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709

GeneID: 919
Latest Publications: View the latest CD247 articles on
UniProt: View information about CD247 on
Keywords: FITC anti-CD247 (TCRζ, CD3ζ), 6B10.2, FITC, CD247, CD3ζ , Human, Intracellular Staining for Flow Cytometry, Immunology, Antibodies

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Related Products

FITC Mouse IgG1, κ Isotype Ctrl (FC)MOPC-21FC
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC

Other Formats

Purified anti-CD247 (TCRζ, CD3ζ)6B10.2WB, ICFC

Related Pathways


Related Categories

Immunology > Human Immunology > NK/NKT > CD247 (TCRζ, CD3ζ)
Cell Biology > Antigens A-Z > TCR zeta chain (CD247)
Cell Biology > Signaling/Signaling Intermediates > TCR zeta chain (CD247)

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-CD247 (TCRζ, CD3ζ)
    Jurkat (1) and Raiji (2)

    Jurkat (1) and Raiji (2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-CD247 (TCR zeta, CD3 zeta) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

  • FITC anti-CD247 (TCRζ, CD3ζ)
    Human peripheral blood lymphocytes surface

    Human peripheral blood lymphocytes surface stained with CD3 (UCHT1) PE then intracellular stained with 6B10.2 FITC

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