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FITC Annexin V
Cat. # Size Price  

640905 25 tests $70.00
    
Reviews (2)
640906 100 tests $165.00
    
Reviews (2)
Product Details

Reactivity: All mammalian species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling: The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.
Excitation
Laser:
Blue Laser (488 nm)
Application
Notes:
Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x10e6 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420403/420404).
5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.
Application
References:
  Publication Library
 Image 1
Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)

 Image 2


Compare all formats

See FITC spectral data





 


Antigen Details

Description: Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer (cat. no. 422201) is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution (cat. no. 420403/420404) or Propidium Iodide Solution (cat. no. 421301). Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
Other Names: Annexin A5
GeneID: 308
UniProt: View information about Annexin V on UniProt.org
Keywords: FITC Annexin V, FITC, Annexin A5 , All mammalian species, Flow Cytometry, Biofunctional Assay, Immunology, Antibodies
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Version: 3 Revision Date: 2013-07-05
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Pacific Blue™ Annexin V Apoptosis Detection Kit with PI
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with LEAF™ purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with Pacific Blue™ Annexin V Apoptosis Detection Kit with PI.





  • Pacific Blue™ Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, stimulated (4 hours) with (bottom) or without (top) LEAF™ purified anti-CD95, clone EOS9.1, then stained with Annexin V Pacific Blue™ and viability dye 7-AAD





  • FITC Annexin V
    Human T leukemia cell line,

    Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with BioLegend’s anti-human CD95 (EOS9.1) mAb (cat. 305704) for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI) (cat. 421301)





  • Alexa Fluor® 647 Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was stimulated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V Alexa Fluor® 647.

  • APC Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V APC.

  • PE Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V PE.

  • FITC Annexin V Apoptosis Detection Kit with PI
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend's LEAF™ purified anti-CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with PI (Cat. No. 640914)





  • FITC Annexin V Apoptosis Detection Kit with 7-AAD
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with BioLegend's LEAF™ purified anti-human CD95 (clone EOS9.1) mAb (Cat. No. 305704) for 4 hours, then stained with FITC Annexin V Apoptosis Detection Kit with 7-AAD (Cat. No. 640922).





  • Purified Annexin V
    Human T cell leukemia cell

    Human T cell leukemia cell line, Jurkat, was treated (4 hours) with (top 2 panels) or without (bottom two panels) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V FITC (panels 1 & 3) or with purified recombinant Annexin V to block Annexin V FITC staining (panels 2 & 4).





  • Brilliant Violet 421™ Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was stimulated (4 hours) with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1), then stained with Annexin V Brilliant Violet 421™.

  • Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with LEAF™ purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD.





  • APC Annexin V Apoptosis Detection Kit with 7-AAD
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with LEAF™ purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with APC Annexin V Apoptosis Detection Kit with 7-AAD.





  • PE Annexin V Apoptosis Detection Kit with 7-AAD
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with LEAF™ purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with PE Annexin V Apoptosis Detection Kit with 7-AAD.





  • Biotin Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1) for 4 hours, then stained with biotinylated Annexin V, followed by Sav-PE.

  • APC Annexin V Apoptosis Detection Kit with PI
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat was treated with (top) or without (bottom) LEAF™ purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with APC Annexin V Apoptosis Detection Kit with PI.





  • PerCP/Cy5.5 Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1) for 4 hours, then stained with Annexin V PerCP/Cy5.5.

  • Brilliant Violet 510™ Annexin V
    Human T-cell leukemia cell line,

    Human T-cell leukemia cell line, Jurkat, was treated with (filled histogram) or without (open histogram) LEAF™ purified anti-CD95 (clone EOS9.1) for 4 hours, then stained with Annexin V Brilliant Violet 510™.

  • Purified anti-Annexin A1
    Lipocortin-1 (74/3) and isotype control

    Lipocortin-1 (74/3) and isotype control staining of K562 cells analyzed by flow cytometry. Cells were pretreated with Fix/Perm buffer. A goat anti-mouse-PE conjugate was used as secondary antibody.

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