Purified anti-Clusterin Antibody

Pricing & Availability
Clone
A15113A (See other available formats)
Other Names
Apolipoprotein J, Apo-J, Complement cytolysis inhibitor, Aging-associated gene 4 protein, Complement-associated protein SP-40,40, Ku70-binding protein 1, NA1/NA2 TRPM-2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15113A_PURE_Clusterin_Antibody_1_WB_011717
Western blot of anti-Clusterin antibody (clone A15113A) and isotype-matched IgG2a control, binding to endogenous and recombinant human clusterin. Lane 1: 0.5 µL of human serum; Lane 2: 50 ng of recombinant human clusterin protein. Blots were incubated with 5 µg/ml of each primary antibody at room temperature for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system.
  • A15113A_PURE_Clusterin_Antibody_1_WB_011717
    Western blot of anti-Clusterin antibody (clone A15113A) and isotype-matched IgG2a control, binding to endogenous and recombinant human clusterin. Lane 1: 0.5 µL of human serum; Lane 2: 50 ng of recombinant human clusterin protein. Blots were incubated with 5 µg/ml of each primary antibody at room temperature for one hour, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system.
  • A15113A_PURE_Clusterin_Antibody_2_IHCP_011717
    IHC staining of anti-Clusterin antibody (clone A15113A) on formalin-fixed paraffin-embedded brain tissue of a patient with Alzheimer's disease. Following antigen retrieval using Sodium Citrate H.I.E.R. and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
See high resolution IHC-P data...
Cat # Size Price Quantity Avail. Save
848701 25 µg 68€
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848702 100 µg 156€
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Description

Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. The protein exhibits a wide array of biological functions including clearance of cellular debris through chaperone activity, apoptosis, lipid transportation, cell clustering, and complement inhibition. Clusterin is involved in many diseases related to oxidative stress and aging, including neurodegenerative disorders, cancers, diabetes, and inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human clusterin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, Direct ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 20 ng/ml - 3.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650695 (BioLegend Cat. No. 848701)
AB_2650696 (BioLegend Cat. No. 848702)

Antigen Details

Structure
Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD.
Distribution

Tissue distribution: Ubiquitously expressed.
Cellular distribution: Primarily extracellular in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Intracellular clusterin is primarily present in the endoplasmic reticulum, cytosol, mitochondrion, and nucleus.

Function
Clusterin is involved in several biological processes including extra- and intracellular chaperone activity, pro- and anti-apoptotic properties, lipid transportation, cell clustering, modulation of NF-kappa-B transcriptional activity, and complement inhibition.
Interaction
HDL.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Apolipoproteins
Antigen References

1. Trougakos IP. 2013. Gerontology 59:514. PubMed
2. Rizzi F, Bettuzzi S. 2010. Endocr. Relat. Cancer 17:R1. PubMed
3. Desikan RS, et al. 2014. JAMA Neurol. 71:180. PubMed
4. Carver JA, et al. 2003. IUBMB Life. 55:661. PubMed

Gene ID
1191 View all products for this Gene ID
UniProt
View information about Clusterin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/17/2017

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