Recombinant Posi-Tag Epitope Tag Protein (Previously Covance catalog# PRO-133P)

Pricing & Availability
Other Names
Posi-Tag
Previously
Covance Catalog# PRO-133P
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recom_Posi-Tag_Bacteria_WB_030515.jpg
Western blot analysis of 0.5 µl/lane of Posi-Tag using HA-HRP (Cat. No. 901519), Myc-HRP (Cat. No. 626803), Glu-Glu (Cat. No. 901801), GST (Cat. No. 640802), His (Cat. No. 913801), HSV (Cat. No. 913801), T7 (Cat. No. 903201), V5 (Cat. No. 903801), VSV (Cat. No. 903901), S-Tag (Cat. No. 688102) and FLAG-HRP (Cat. No. 637311) antibodies.
  • recom_Posi-Tag_Bacteria_WB_030515.jpg
    Western blot analysis of 0.5 µl/lane of Posi-Tag using HA-HRP (Cat. No. 901519), Myc-HRP (Cat. No. 626803), Glu-Glu (Cat. No. 901801), GST (Cat. No. 640802), His (Cat. No. 913801), HSV (Cat. No. 913801), T7 (Cat. No. 903201), V5 (Cat. No. 903801), VSV (Cat. No. 903901), S-Tag (Cat. No. 688102) and FLAG-HRP (Cat. No. 637311) antibodies.
  • recom_Posi-Tag_Bacteria_WB_2_030515.jpg
    Western blot showing several of our epitope tag antibodies and their detection of 1 µg of Posi-Tag control protein. All of the monoclonals were used at a 1:1,000 dilution. Poly9248 (Cat. No. 924801) was used at a dilution of 1:50,000.
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931301 50 µl £75
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Description

Posi-Tag is a recombinant protein that contains 12 different tags. It has a predicted weight of 45 kDa and is intended for use as a positive control in western blot applications. The lysate contains the commonly used epitope tags HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, and FLAG™ tag.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Bacterial lysate in loading buffer.
Preparation
1 x reduced SDS-PAGE loading buffer.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the protein. May be stored at 2-8°C for short periods.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This protein is effective as a positive control for epitope tag antibodies in immunoblotting.

Our Posi-Tag protein lysate contains the commonly used epitope tags: HA, c-myc, Glu-Glu, GST, 6-His, HSV, T7, V5, VSV, E-tag, S-tag, FLAG (TM). These proteins were fused in the following order, GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, and 6-His.

*Predicted MW = ~45 kD
FLAG is a trademark of Sigma-Aldrich Co.

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about Posi-Tag on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 03/27/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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