Purified anti-Tau Phospho (Ser396) Antibody (Previously Covance catalog# MMS-546P)

Pricing & Availability
Clone
PHF-13 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Previously
Covance Catalog# MMS-546P
Isotype
Mouse IgG2b
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Product Citations
publications
PHF-13_Pure_tau-phospho-ser396_Antibody_1_062218
IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • PHF-13_Pure_tau-phospho-ser396_Antibody_1_062218
    IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • PHF-13_Pure_tau-phospho-ser396_Antibody_2_062218
    IHC staining of purified anti-Tau Phospho (Ser396) antibody (clone PHF-13) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
829003 25 µl £81
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829001 100 µl £204
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Description

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated tau protein. Predominantly expressed in axons, alternatively spliced forms of tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-tau differs from normal tau by its abnormal hyperphosphorylation, which results in decreased binding of tau to microtubules. The decreased affinity of PHF-tau for microtubules, coupled with reduced levels of normal tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of tau is believed to be a key event in the pathogenesis of Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The PHF-13 antibody was raised using PHF-tau preparations from human brain.
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective informalin-fixed paraffin-embedded immunohistochemical staining (IHC-P) and immunoblotting (WB).

The PHF-13 antibody reacts with human tau phosphorylated at Ser 396. 

Application References

(PubMed link indicates BioLegend citation)
  1. Lasagna-Reeves CA, et al. 2012. FASEB J. (26):1946 (WB) (PUBMED)
  2. Lauretti E, et al. 2017. Ann Clin Transl Neurol. 4(8): 564 (WB, IHC)
  3. Li JG, et al. 2017. Sci Rep. (7):46002 (WB, IHC)
  4. Li JG, et al. 2014. Ann Neurol. 75(6):851 (WB, IHC)
RRID
AB_2728540 (BioLegend Cat. No. 829003)
AB_2564925 (BioLegend Cat. No. 829001)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho (Ser396) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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