Purified anti-FOX3 (NeuN) Antibody (Previously Covance catalog# SIG-39860)

Pricing & Availability
Clone
1B7 (See other available formats)
Other Names
RNA binding protein fox-1 homolog 3, fox-1 homolog C, neuronal nuclei, hexaribonucleotide binding protein 3
Previously
Covance Catalog# SIG-39860
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1_1B7_PURE_FOX3_NeuN_Antibody_IHC_1_010418
IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 4X objective. Scale bar: 300 µm
  • 1_1B7_PURE_FOX3_NeuN_Antibody_IHC_1_010418
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 4X objective. Scale bar: 300 µm
  • 2_1B7_PURE_FOX3_NeuN_Antibody_IHC_2_010418
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 4X objective. Scale bar: 300 µm
  • 3_1B7_PURE_FOX3_NeuN_Antibody_IHC_3_010418
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 4_1B7_PURE_FOX3_NeuN_Antibody_IHC_4_010418
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 100 µm
  • 5_1B7_PURE_FOX3_NeuN_Antibody_WB_010418
    Western blot of purified anti-FOX3 (NeuN) antibody (clone 1B7). Lane 1: Molecular weight marker; Lane 2: 10 µg of mouse brain lysate; Lane 3: 10 µg of rat brain lysate. The blots were incubated with 0.05 µg/mL of clone 1B7 or mouse IgG2a, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • 6_MCS-1B7_Purified_FOX3slashNeuN_Antibody_IHC_011615
    IHC staining of purified anti-FOX3 (NeuN) antibody (clone 1B7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
834502 25 µl £64
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834501 100 µl £136
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Description

Fox3 is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. Fox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The 1B7 antibody reveals strong nuclear and distal cytoplasmic staining for Fox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This Fox3/NeuN antibody is therefore an excellent marker of neuronal cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
Formulation
Phosphate-buffered solution + 10mM sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P -  Quality tested
WB - Validated
ICC - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The double band observed in the immunoblot corresponds to the two NeuN isoforms produced by alternative splicing with expected molecular weights of 46 kD and 48 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Herculano-Houzel S and Lent R. 2005. J Neurosci. 25:2518-21. (ICC)
  2. Kim KK, et al. 2009. J Biol Chem. 284:31052-31061. (WB, ICC, IHC-P)
  3. Underwood JG, et al. 2005. Mol Cell Biol. 25:10005-10016. (IHC-F, WB)
RRID
AB_2734601 (BioLegend Cat. No. 834502)
AB_2564991 (BioLegend Cat. No. 834501)

Antigen Details

Structure
Expected MW: 46 kD and 48 kD
Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Nuclear Markers
Gene ID
146713 View all products for this Gene ID
UniProt
View information about FOX3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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