The BioLegend ELISA Coating Buffer(5X) must be diluted to 1X working solution with D.I. water prior to ELISA coating procedures. e.g. Dilute one (1) part ELISA Coating Buffer(5X) to four (4) parts D.I. water. Each lot of BioLegend ELISA Coating Buffer (5X) has been tested with cytokine ELISA capture antibodies.
