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DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity) Antibody
DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity) Antibody
405410 100 µg €72     
Product Details
Clone: Poly4054
Isotype: Goat Ig
Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with DyLight™ 594 under optimal conditions. The solution is free of unconjugated DyLight™ 594.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

IF
Recommended Usage:

Each lot of this fluorochrome conjugated antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 594  has a maximum absorption of 592nm and a maximum emission of 617 nm (Alexa Fluor® 594 or Texas Red equivalent). Prior to using DyLight™594 conjugates, please verify your instrument's capability of exciting and detecting the fluorochrome.
COA:
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Application
Notes:

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.
Application
References:

1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
2. Tokarev AA, et al. 2011. J. Virol. 85:51. PubMed
3. Wu Z, et al. 2012. Blood 119:2422. PubMed.

Immortalized murine bone marrow-derived macrophages
Immortalized murine bone marrow-derived macrophages stimulated overnight with LPS were stained with Atto-488 phalloidin (green) and purified TNF-α (clone MP6-XT22), secondarily stained with Goat anti-Rat IgG Dylight 594 (red). Data provided by James Harris, Trinity College.


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Description:

Goat anti-rat IgG antibody reacts with the heavy chains of rat IgG and the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
GeneID: 295279
Latest Publications: View the latest IgG articles on HighwirePress.com
UniProt: View information about IgG on UniProt.org
Keywords: DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity), Poly4054, DyLight™ 594, Rat, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE/Cy7 Goat anti-rat IgG (minimal x-reactivity)
    C57BL/c mouse splenocytes stained with

    C57BL/c mouse splenocytes stained with purified rat anti-mouse CD45R/B220 (RA3-6B2), followed anti-rat IgG (Poly4054) PE/Cy7

  • DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity)
    Immortalized murine bone marrow-derived macrophages

    Immortalized murine bone marrow-derived macrophages stimulated overnight with LPS were stained with Atto-488 phalloidin (green) and purified TNF-α (clone MP6-XT22), secondarily stained with Goat anti-Rat IgG Dylight 594 (red). Data provided by James Harris, Trinity College.

  • DyLight™ 405 Goat anti-rat IgG (minimal x-reactivity)
    Mitotic 293E cell was stained

    Mitotic 293E cell was stained with Purified anti-Histone H3-Phosphorylated (Ser28) Antibody (clone HTA28) and labeled with DyLight™ 405 anti-rat IgG (clone Poly4054 (blue)). Cytosol indicated by Alexa Fluor® 488 anti-Tubulin-alpha (clone 10D8 (green)) and nucleus by PI (red).

  • Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity)
    293E cells were stained with

    293E cells were stained with purified anti-Histone H3-Phospho (Ser28) antibody (clone HTA28) and labeled with BV421™ anti-rat IgG (clone Poly4054)(blue). Cytosol is indicated by Alexa Fluor® 488 anti-tubulin-alpha (clone 10D8)(green) and nuclei are stained by propidium iodide. HTA28 only stains mitotic cells (violet).

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