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DyLight™ 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody
DyLight™ 594 Goat anti-mouse IgG (minimal x-reactivity) Antibody
405311 100 µg €72     
Product Details
Clone: Poly4053
Isotype: Goat Ig
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with DyLight™ 594 under optimal conditions. The solution is free of unconjugated DyLight™ 594
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

IF - Reported in the literature
Recommended Usage:

Each lot of this fluorochrome conjugated antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 594  has a maximum absorption of 592nm and a maximum emission of 617 nm (Alexa Fluor® 594 or Texas Red equivalent). Prior to using DyLight™594 conjugates, please verify your instrument's capability of exciting and detecting the fluorochrome.
COA:
Enter Lot#:   
Application
Notes:

This DyLight™ 594 conjugated polyclonal goat anti-mouse IgG antibody is useful for immunofluorescent staining for microscopy.
Hela cells were stained with
Hela cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight594™ anti-mouse IgG (Poly4053).


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Description:

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of mouse immunoglobulins.
GeneID: 16059
Latest Publications: View the latest IgG articles on HighwirePress.com
UniProt: View information about IgG on UniProt.org
Keywords: DyLight™ 594 Goat anti-mouse IgG (minimal x-reactivity), Poly4053, DyLight™ 594, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE/Cy7 Goat anti-mouse IgG (minimal x-reactivity)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified mouse anti-human CD3 (UCHT1), followed by anti-mouse IgG (Poly4053) PE/Cy7

  • PerCP/Cy5.5 Goat anti-mouse IgG (minimal x-reactivity)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified mouse anti-human CD3 (UCHT1), followed by anti-mouse IgG (Poly4053) PerCP/Cy5.5

  • DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity)
    Hela cells were stained with

    Hela cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight488™ anti-mouse IgG (Poly4053).

  • DyLight™ 594 Goat anti-mouse IgG (minimal x-reactivity)
    Hela cells were stained with

    Hela cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight594™ anti-mouse IgG (Poly4053).

  • APC/Cy7 Goat anti-mouse IgG (minimal x-reactivity)
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified mouse anti-human CD3(UCHT1), followed by anti-mouse IgG APC/Cy7

  • DyLight™ 405 Goat anti-mouse IgG (minimal x-reactivity)
    293E cells were stained with

    293E cells were stained with purified anti-Tubulin-alpha (clone TU-01) and labeled with DyLight™ 405 anti-mouse IgG (clone Poly4053 (blue)). Nuclei were stained with PI mounting medium (red).

  • Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity)
    293E cells were stained with

    293E cells were stained with purified anti-tubulin-alpha (clone TU-01) and then BV421™ anti-mouse IgG (clone Poly4053)(blue) as the secondary antibody. Nuclei were stained with propidium iodide (red).

    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with purified mouse anti-human CD3 (UCHT1), followed by anti-mouse IgG (Poly4053) Brilliant Violet 421™.

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