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DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody
DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody
406404 100 µg €72     
Product Details
Clone: Poly4064
Isotype: Donkey Ig
Reactivity: Rabbit
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions. The solution is free of unconjugated DyLight™ 488.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IF - Reported in the literature
Recommended Usage:

Each lot of this fluorochrome conjugated antibody is quality control tested by flow cytometry. It is recommended that the reagent be titrated for optimal performance for each application.

* DyLight™ 488  has a maximum absorption of 493nm and a maximum emission of 518 nm (similar to Alexa Fluor® 488 or FITC).
COA:
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Application
Notes:

This DyLight™ 488 conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.
293T cells were stained with
293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).


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Description:

This polyclonal Donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.
GeneID: 100343867
Latest Publications: View the latest Rabbit IgG articles on HighwirePress.com
UniProt: View information about Rabbit IgG on UniProt.org
Keywords: DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity), Poly4064, DyLight™ 488, Rabbit, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Brilliant Violet 421™ Donkey anti-rabbit IgG (minimal x-reactivity)
    293E cells were stained by

    293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with BV421 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with propidium iodide (red).

  • DyLight™ 488 Donkey anti-rabbit IgG (minimal x-reactivity)
    293T cells were stained with

    293T cells were stained with anti-Tubulin-γ and secondarily labeled with DyLight™ 488 donkey anti-rabbit IgG (clone Poly4064).

  • DyLight™ 405 Donkey anti-rabbit IgG (minimal x-reactivity)
    293E cells were stained by

    293E cells were stained by purified anti-Tubulin-gamma (clone Poly6209) and labeled with DyLight™ 405 anti-rabbit-IgG (clone Poly4064 (blue)). Nuclei were stained with PI mounting medium (red).

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