BioLegend Direct-Blot
  Introduction   Supplemental Data   Products  
The most common practice when performing a western blot is to use a secondary antibody to detect the proteins of interest. This is advantageous if there is a need to amplify the signal. However, if the target under study is abundant, and the quality of a directly conjugated primary antibody to Horse Radish Peroxidase (HRP) is high, there is really no advantage or need to use a secondary conjugated antibody. Thus, we are happy to introduce Direct-Blot™, primary antibodies directly conjugated to HRP, to help you speed up, simplify and reduce the cost of your experiment.
This content requires opt in of marketing cookies.
Open with YouTubeChange your cookie preferences
Summary of advantages:
  • Shorter protocol
  • Highly sensitive
  • Excellent stability
  • Easier to multiplex
  • Easier to combine with Immunoprecipitation
  • No potential background from HRP conjugated secondary antibodies
Forgot your password? Reset Password
Request an Account