PE anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
7 reviews
Product Citations
publications
IM7_PE_071608.jpg
C57BL/6 mouse splenocytes stained with IM7 PE
  • IM7_PE_071608.jpg
    C57BL/6 mouse splenocytes stained with IM7 PE
  • IM7_PE_human
    Human peripheral blood lymphocytes stained with IM7 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
103023 25 tests 28€
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103007 50 µg 36€
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103024 100 tests 76€
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103008 200 µg 96€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For µg sizes, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been validated for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  2. Morley S, et al. 2008. J Immunol. 181:4696. PubMed
  3. Chen Y, et al. 2010. Mol Cancer Ther. 2.374305556. PubMed
  4. Sentman A 2011. Blood. 117:6571. PubMed
  5. Lee Y, et al. 2012. Nat Immunol. 13:991. PubMed
  6. Blacking T, et al. 2011. Vet Immunol Immunopathol. 141:46. PubMed
  7. Shao W, et al. 2010. Transplant Proc. 42:2777. PubMed
  8. Tyne A, et al. 2013. Vaccine. 31:4322. PubMed
  9. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  10. Davila S, et al. 2014. J Immunol. 192:4284. PubMed
  11. Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
  12. Zhu E, et al. 2014. J Immunol. 192:5599. PubMed
  13. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  14. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  15. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  16. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  17. Fiume G, et al. 2015. Sci Rep. 5: 13864. PubMed
  18. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  19. Kaji T, et al. 2016. Int Immunol. 28: 267 - 282. PubMed
  20. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  21. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  22. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  23. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  24. M H, et al. 2016. Open Bio. 6: 150208. PubMed
  25. M H, et al. 2016. Cell Transplant. 25: 811-27. PubMed
  26. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  27. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  28. Hammer A, et al. 2016. Proc Natl Acad Sci U S A. 113(49):14109-14114. PubMed
  29. Li P, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.24. PubMed
  30. Trial J, et al. 2017. Basic Research in Cardiology. 10.1007/s00395-017-0623-4. PubMed
  31. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
  32. Hammer A, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0954-y. PubMed
  33. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  34. Zhao J, et al. 2018. Stem Cell Reports. 10:180. PubMed
  35. Li P, et al. 2018. J Cell Mol Med. 22:3423. PubMed
  36. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  37. Fan MY 2018. Cell reports. 25:1204. PubMed
  38. Biton M 2018. Cell. 175:1307. PubMed
  39. Wang D 2018. Immunity. 48:659. PubMed
Publication Library
RRID
AB_493686 (BioLegend Cat. No. 103023)
AB_312958 (BioLegend Cat. No. 103007)
AB_493687 (BioLegend Cat. No. 103024)
AB_312959 (BioLegend Cat. No. 103008)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
Leukocytes, Epithelial cells, Endothelial cells, Mesenchymal cells, Mesenchymal Stem Cells, B cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 10.15.2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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