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BioLegend Brillaint Violet Antibody Conjugates

Brilliant Violet™ antibody conjugates, proudly co-developed by BioLegend and Sirigen, are an innovative class of novel research reagents, providing more options for your multicolor flow cytometry panels and getting you better results. Maximize the capacity of your violet laser with our large selection of directly labeled Brilliant Violet™ antibody conjugates.

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Features
• Extremely Bright
• Stable to Fixation
• Effective for Surface and Intracellular Antigens
• Exceptional Photostability
• Discrete Excitation/Emission Spectra
• No Special Disposal Requirements
• Nontoxic - for Sorting or Live Cell Imaging
• Effective Across a Wide pH Range
• No Special Buffers Required
• Large Selection of Antibody Specificities
• Excellent Pricing
Brilliant Violet™ Family
Brilliant Violet 421™ | Brilliant Violet 510™ | Brilliant Violet 570™ | Brilliant Violet 605™ | Brilliant Violet 650™ | Brilliant Violet 711™ | Brilliant Violet 785™
Brilliant Violet 421™
The first in the series, BV421™ can increase assay sensitivity by logarithmic orders of magnitude without increasing background or spillover, making it ideal for detecting rare cell populations or weakly expressed cell markers. It is exceptionally photostable, enabling the visualization of antigens with directly conjugated antibodies for confocal microscopy applications. Est. MW = 70 kD.

Excitation Max = 405 nm, Emission Max = 421 nm

Recommended filter = 450/50

Comparable Fluorophores: Pacific Blue™, Alexa Fluor® 405, eFluor® 450, BD Horizon™ V450, Cascade Blue™

Brightness = 5 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,500,000 M-1cm-1

Quantum Yield = 0.65 in DPBS

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Brilliant Violet 510™
Brilliant Violet 510™ is a novel non-tandem polymer with excellent signal-to-noise, excited by the violet laser. It can provide dramatic improvements over existing fluorophores emitting in this range such as Pacific Orange™, AmCyan, and Horizon™ V500. Est. MW = 77 kD.

Excitation Max = 405 nm, Emission Max = 510 nm

Recommended filter = 510/50

Comparable Fluorophores: BD Horizon™ V500, Pacific Orange™, Cascade Yellow™, AmCyan

Brightness = 3 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 577,000 M-1cm-1

Quantum Yield = 0.44 in DPBS

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Brilliant Violet 570™
Brilliant Violet 570™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to Pacific Orange™ for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.

Excitation Max = 405 nm, Emission Max = 570 nm

Recommended filter = 585/42

Comparable Fluorophores: Pacific Orange™, Cascade Yellow™, Qdot® 545, Qdot® 565, eFluor® 565NC

Brightness = 3 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,300,000 M-1cm-1

Quantum Yield = 0.08 in DPBS

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Brilliant Violet 605™
Brilliant Violet 605™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 605NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.

Excitation Max = 405 nm, Emission Max = 603 nm

Recommended filter = 610/20

Comparable Fluorophores: Qdot® 605, eFluor® 605NC

Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,400,000 M-1cm-1

Quantum Yield = 0.29 in DPBS

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Brilliant Violet 650™
Brilliant Violet 650™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 650NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 63 kD.

Excitation Max = 405 nm, Emission Max = 645 nm

Recommended filter = 660/20

Comparable Fluorophores: Qdot® 655, eFluor® 650NC

Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,500,000 M-1cm-1

Quantum Yield = 0.17 in DPBS

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Brilliant Violet 711™
Brilliant Violet 711™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides a much brighter alternative to eFluor® 700NC for multicolor flow on the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 70 kD.

Excitation Max = 405 nm, Emission Max = 711 nm

Recommended filter = 710/50

Comparable Fluorophores: eFluor® 700NC, Qdot® 705

Brightness = 4 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,800,000 M-1cm-1

Quantum Yield = 0.15 in DPBS

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Brilliant Violet 785™
Brilliant Violet 785™ is a novel molecule based on the Brilliant Violet 421™ polymer core. It provides further options for the violet laser and is a better alternative to nanocrystals for intracellular flow cytometry. Est. MW = 60 kD.

Excitation Max = 405 nm, Emission Max = 785 nm

Recommended filter = 780/60

Comparable Fluorophores: QDot® 800

Brightness = 3 (On a scale from 1 to 5, with 5 being the brightest.)

Molar Extinction Coeff. = 2,500,000 M-1cm-1

Quantum Yield = 0.04 in DPBS

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Learn the answers to Frequently Asked Questions about Brilliant Violet™ conjugates…
Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 510™, BV510™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, BV650™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™, and BV785™ are trademarks of Sirigen Group Ltd.
View the full listing of trademarks here.
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