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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Recent studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
Other Names:
IL-7 receptor α chain, IL-7Rα
Structure:
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution:
Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function:
T cell and immature B cell proliferation and development
Ligand Receptor:
IL-7
Antigen References:
1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with purified CD127 (clone A019D5) (filled histogram) or mouse IgG1 isotype control (open histogram), followed by anti-mouse IgG FITC.
PE anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) PE (top) or mouse IgG1 PE isotype control (bottom).
Pacific Blue™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 PE and CD127 (clone A019D5) Pacific Blue™ (top) or mouse IgG1 Pacific Blue™ isotype control (bottom).
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
FITC anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) FITC (top) or mouse IgG1 FITC isotype control (bottom).
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 APC and CD127 (clone A019D5) Alexa Fluor® 488 (top) or mouse IgG1 Alexa Fluor® 488 isotype control (bottom).
APC anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) APC (top) or mouse IgG1 APC isotype control (bottom).
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom).
PE/Cy7 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cy7 (top) or mouse IgG1 PE/Cy7 isotype control (bottom).
PerCP/Cy5.5 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PerCP/Cy5.5 (top) or mouse IgG1 PerCP/Cy5.5 isotype control (bottom).
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
Human RBC-lysed whole blood cells were stained with anti-CD127 (clone A019D5) BV570™ and anti-CD25 (clone BC96) BV421™. Cells were gated on the lymphocyte population.
PE/Cy5 anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cy5 (top) or mouse IgG1, κ PE/Cy5 isotype control (bottom).
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 650™.
