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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 605™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Other Names:
αX integrin, integrin αX chain, CR4, p150
Structure:
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution:
dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function:
Cellular adhesion
Ligand Receptor:
iC3b, fibrinogen
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press. 2. Springer TA. 1994. Cell 76:301. 3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 APC and PK136 PE
Biotin anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE
FITC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
PE anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)
Purified anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and Alexa Fluor® 488 N418 (top) or Alexa Fluor® 488 Armenian hamster IgG isotype control (bottom)
Alexa Fluor® 647 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647
Fixed whole mount mouse epidermis Langerhans cells were stained with Alexa Fluor® 647 CD11c (red) (clone N418) and PE CD207 (yellow) (clone 4C7). Isotype controls at the same concentrations were used for the negative control (bottom image). Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.
PE/Cy5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy5 N418 (top) or PE/Cy5 Armenian hamster IgG isotype control (bottom)
PE/Cy7 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy7 N418 (top) or PE/Cy7 Armenian hamster IgG isotype control (bottom)
Brilliant Violet 605™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 605™.
Alexa Fluor® 700 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 700 and M5/114.15.2 PE
Pacific Blue™ anti-mouse CD11c
C57BL/6 mouse splenocytes stained with Pacific Blue™ N418
APC/Cy7 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cy7 (top) or Armenian hamster IgG APC/Cy7 isotype control (bottom).
PerCP/Cy5.5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP/Cy5.5
PerCP anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP and M5/114.15.2 PE
Brilliant Violet 421™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
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Brilliant Violet 570™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 570™ (top) or Armenian hamster IgG Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 785™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 785™.
Brilliant Violet 510™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E APC and CD11c (clone N418) Brilliant Violet 510™ (top) or Armenian hamster IgG Brilliant Violet 510™ isotype control (bottom).
