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Brilliant Violet 570™ Streptavidin
Brilliant Violet 570™ Streptavidin
405227 100 µl $200.00     
Product Details
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: This streptavidin was conjugated with Brilliant Violet 570™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 570™ and unconjugated streptavidin.
Concentration: 0.1 mg/ml
Storage & Handling: The streptavidin solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

Each lot of this Streptavidin-Brilliant Violet 570™ is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
Enter Lot#:   
 Human peripheral blood lymphocytes were
Human peripheral blood lymphocytes were stained with biotinylated CD3 (clone UCHT1) (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 570™.


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Description:

Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 570™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 570™ by 405 nm laser light induces a fluorescence maximum emission of 570 nm.
Other Names: SAv-Brilliant Violet 570™
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: Brilliant Violet 570™ Streptavidin, Brilliant Violet 570™, SAv-Brilliant Violet 570™ , Mouse, Human, Rat, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
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    C57BL/6 splenocytes stained with biotinylated

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    Induction of IL-6 in human

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    Induction of mouse IL-17A in

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  • LEGEND MAX™ Human IL-21 ELISA Kit with Pre-coated Plates




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    Mouse IL-6 induced by mIL-17A

    Mouse IL-6 induced by mIL-17A in fetal mouse skin fibroblasts.

  • LEGEND MAX™ Human anti-mouse Ig (HAMA) ELISA Kit with Pre-coated Plates




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    CTLL-2 proliferation induced by mouse

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    MC/9 cell proliferation induced by

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    Induction of IL-6 in human

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    Rat TNF-α cytotoxicity on L929

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    Chemotactic activity of human RANTES.

    Chemotactic activity of human RANTES.

  • Recombinant Mouse IL-17A/F Heterodimer (carrier-free)
    IL-6 induced by mIL-17A/IL-17F on

    IL-6 induced by mIL-17A/IL-17F on fetal mouse skin fibroblasts.

  • Recombinant Mouse VEGF120 (carrier-free)
    VegfA induces the proliferation of

    VegfA induces the proliferation of HUVEC cells

  • Recombinant Mouse CCL2 (MCP-1) (carrier-free)




  • Recombinant Human PDGF-BB (carrier-free)




  • Recombinant Human CCL2 (MCP-1) (carrier-free)
    THP-1 cell chemotaxis induced by

    THP-1 cell chemotaxis induced by human MCP-1.

  • Recombinant Mouse M-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by mouse M-CSF.

  • Recombinant Human CXCL12 (SDF-1α) (carrier-free)
    Bioassay for human SDF-1

    Bioassay for human SDF-1

  • Recombinant Mouse IL-3 (carrier-free)
    Bioactivity of mouse IL-3 was

    Bioactivity of mouse IL-3 was tested by a proliferation assay using M-NFS-60 cells.

  • Recombinant Human GM-CSF (carrier-free)
    TF-1 cell proliferation induced by

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  • Recombinant Mouse SCF (carrier-free)
    TF-1 cell proliferation induced by

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    CXCL1 induction in human fibroblast

    CXCL1 induction in human fibroblast by human IL-17F

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    Human TruStain FcX™ treated (filled

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  • Recombinant Mouse IL-12 (p70) (carrier-free)
    Mouse splenocytes IFNγ production induced

    Mouse splenocytes IFNγ production induced by mouse IL-12.

  • Recombinant Mouse IL-17F (carrier-free)
    CXCL1 induced by mouse IL-17F

    CXCL1 induced by mouse IL-17F in fetal mouse skin fibroblasts

  • Recombinant Human IL-12 (p70) (carrier-free)
    IFNγ induced by human IL-12

    IFNγ induced by human IL-12 in PBMC activated with PHA

  • Recombinant Mouse IL-25 (IL-17E) (carrier-free)
    Mouse IL-25 binding to recombinant

    Mouse IL-25 binding to recombinant IL17BR/Fc Chimera detected by functional ELISA

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  • Recombinant Mouse IL-15 (carrier-free)
    CTLL-2 cell proliferation induced by

    CTLL-2 cell proliferation induced by mouse IL-15

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    Mouse IXN/2B cell proliferation induced

    Mouse IXN/2B cell proliferation induced by human IL-7

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    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human IL-4

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    Mouse splenocytes IL-17A secretion induced

    Mouse splenocytes IL-17A secretion induced by human IL-23. Bioassay activity for human IL-23.

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    Human IL-8 bioactivity

    Human IL-8 bioactivity

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    CTLL-2 cell proliferation induced by

    CTLL-2 cell proliferation induced by human IL-2.

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    Inhibition of IL-2 production induced

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  • Recombinant Mouse IL-12 p40 Homodimer (carrier-free)
    Mouse IL-12 p40 homodimer is

    Mouse IL-12 p40 homodimer is able to inhibit IL-12-dependent IFNγ production.

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    Inhibition of IL-2 production by

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    M-NSF-60 cell proliferation induced by

    M-NSF-60 cell proliferation induced by mouse IL-34.

  • LEGEND MAX™ Human IL-1β ELISA Kit with Pre-coated Plates




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  • Brilliant Violet 421™ Streptavidin
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  • Recombinant Mouse TACI-Fc Chimera (carrier-free)
    Inhibition of BAFF mediated B

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  • Recombinant Mouse IL-7 (carrier-free)
    PHA activated PBL proliferation induced

    PHA activated PBL proliferation induced by mouse IL-7.

  • LEGEND MAX™ Mouse FGL2 ELISA Kit with Pre-coated Plates




  • Recombinant Human IL-34 (carrier free)
    Monocyte cell proliferation induced by

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  • RBC Lysis/Fixation Solution (10X)
    Human peripheral blood cells were

    Human peripheral blood cells were stained with anti-human CD3 PerCP/Cy5.5 and anti-human CD4 FITC; red blood cells were lysed with BioLegend RBC Lysis/Fixation Solution.





  • Recombinant Mouse CXCL9 (MIG) (carrier-free)
    T cells chemoattracted by mouse

    T cells chemoattracted by mouse CXCL9.

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    TF-1 cell proliferation induced by

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  • Human Mesothelin ELISA MAX™ Deluxe




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    CTLL-2 cell proliferation induced by

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  • LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CXCL5 (GCP-2) (carrier-free)
    Human neutrophils chemoattracted by mouse

    Human neutrophils chemoattracted by mouse CXCL5.

  • Recombinant Human CXCL5 (ENA-78) (carrier-free)
    Neutrophils chemoattracted by human CXCL5.

    Neutrophils chemoattracted by human CXCL5.

  • Recombinant Human CXCL10 (IP-10) (carrier-free)
    Human activated T cells chemoattracted

    Human activated T cells chemoattracted by CXCL10.

  • LEGEND MAX™ Human IL-17A/F ELISA Kit with Pre-coated Plates




  • Recombinant Mouse CXCL10 (IP-10) (carrier-free)
    Activated T cells chemoattracted by

    Activated T cells chemoattracted by mouse CXCL10.

  • Recombinant Mouse CXCL1 (KC) (carrier-free)
    Human neutrophils chemoattracted by mouse

    Human neutrophils chemoattracted by mouse CXCL1.

  • Recombinant Human SCF (carrier-free)
    TF-1 cell proliferation induced by

    TF-1 cell proliferation induced by human SCF.

  • Recombinant Mouse G-CSF (carrier-free)
    M-NFS-60 cell proliferation induced by

    M-NFS-60 cell proliferation induced by mouse G-CSF.

  • Recombinant Human CXCL13 (carrier-free)
    Human CXCR5 transfectants chemoattracted by

    Human CXCR5 transfectants chemoattracted by human CXCL13.

  • Recombinant Human M-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by human M-CSF.

  • Recombinant Human CXCL11 (ITAC) (carrier-free)
    CXCR3 transfected BaF3 cells chemoattracted

    CXCR3 transfected BaF3 cells chemoattracted by hCXCL11.

  • Recombinant Mouse CXCL11 (ITAC) (carrier-free)
    CXCR3 transfected BaF3 cells chemoattracted

    CXCR3 transfected BaF3 cells chemoattracted by mCXCL11.

  • Recombinant Mouse CCL2 (MCP-1) (carrier-free)
    THP-1 cells chemoattracted by mouse

    THP-1 cells chemoattracted by mouse MCP-1.

  • Recombinant Human G-CSF (carrier-free)
    M-NFS60 cell proliferation induced by

    M-NFS60 cell proliferation induced by human G-CSF.

  • Recombinant Mouse CXCL12 (SDF-1α) (carrier-free)
    Activated human T cells chemoattracted

    Activated human T cells chemoattracted by mouse CXCL12.

  • LEGEND MAX™ Human Soluble DcR3/TNFRSF6B ELISA Kit with Pre-coated Plates




  • Human MCP-1/CCL2 ELISA MAX™ Deluxe




  • Recombinant Mouse CCL17 (TARC) (carrier-free)
    Human CCR4-Baf3 cells attracted by

    Human CCR4-Baf3 cells attracted by mouse CCL17.

  • Recombinant Mouse IL-5 (carrier-free)
    BCL-1 cell proliferation induced by

    BCL-1 cell proliferation induced by mouse IL-5.

  • LEGEND MAX™ Mouse Isthmin ELISA Kit with Pre-coated Plates




  • Recombinant Human CCL8 (MCP-2) (carrier-free)
    THP-1 cells chemoattracted by human

    THP-1 cells chemoattracted by human CCL8 (MCP-2).

  • Recombinant Mouse CCL8 (MCP-2) (carrier-free)
    THP-1 cells chemoattracted by mouse

    THP-1 cells chemoattracted by mouse CCL8 (MCP-2).

  • Brilliant Violet 605™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 605™.

  • Brilliant Violet 650™ Streptavidin
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with biotinylated CD3 (filled histogram) or mouse IgG1 isotype control (open histogram), followed with Sav-Brilliant Violet 650™.

  • Recombinant Human IL-33 (carrier-free)
    D10.G4.1 cell proliferation induced by

    D10.G4.1 cell proliferation induced by human IL-33 and co-stimulated with anti-CD3.

  • Recombinant Human IL-7 (carrier-free)
    Proliferation of PHA-activated human PBL

    Proliferation of PHA-activated human PBL induced by human IL-7.

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