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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 570™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The RM4-5 antibody blocks the binding of GK1.5 antibody (Cat. No. 100402) and H129.19 (Cat. No. 130302) to CD4 T cells, but not RM4-4 antibody (116002). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100520).
Application References:
1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete) 2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC) 3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. 4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC) 5. León-Ponte M, et al. 2007. Blood 109:3139. (FC) 6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC) 7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed 8. Shigeta A, et al. 2008. Blood 112:4915. PubMed 9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed 10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD4 (clone RM4-5) Brilliant Violet 570™ (top), or rat IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.
Other Names:
L3T4, T4
Structure:
Ig superfamily, 55 kD
Distribution:
Majority of thymocytes, T cell subset
Function:
TCR co-receptor, T cell activation
Ligand Receptor:
MHC class II molecule
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Biotin anti-mouse CD4
C57BL/6 mouse splenocytes stained with biotinylated CD4 (clone RM4-5) (filled histogram) or biotinylated rat IgG2a, κ isotype control (open histogram).
FITC anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) FITC (solid line) or rat IgG2a, κ FITC isotype control (dotted line).
LEAF™ Purified anti-mouse CD4
C57BL/6 mouse splenocytes stained with LEAF™ purified CD4 (clone RM4-5) (filled histogram) or LEAF™ purified rat IgG2a, κ isotype control (open histogram).
PE anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
PE/Cy5 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PE/Cy5 (filled histogram) or rat IgG2a, κ PE/Cy5 isotype control (open histogram).
Purified anti-mouse CD4
C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram).
PE/Cy7 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PE/Cy7 (filled histogram) or rat IgG2a, κ PE/Cy7 isotype control (open histogram).
APC/Cy7 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC/Cy7 (filled histogram) or rat IgG2a, κ APC/Cy7 isotype control (open histogram).
Alexa Fluor® 647 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Alexa Flour® 647 (filled histogram) or rat IgG2a, κ Alexa Flour® 647 isotype control (open histogram).
Alexa Fluor® 488 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Alexa Flour® 488 (solid line) or rat IgG2a, κ Alexa Flour® 488 isotype control (dotted line).
Pacific Blue™ anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Pacific Blue™ (filled histogram) or rat IgG2a, κ Pacific Blue™ isotype control (open histogram).
Alexa Fluor® 700 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Alexa Flour® 700 (filled histogram) or rat IgG2a, κ Alexa Flour® 700 isotype control (open histogram).
PerCP anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PerCP (filled histogram) or rat IgG2a, κ PerCP isotype control (open histogram).
PerCP/Cy5.5 anti-mouse CD4
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) PerCP/Cy5.5 and CD3 PE.
Brilliant Violet 421™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD3 APC and CD4 (clone RM4-5) Brilliant Violet 421™ (top), or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD4 (clone RM4-5) Brilliant Violet 570™ (top), or rat IgG2a, κ Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 605™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD3 PE and CD4 (clone RM4-5) Brilliant Violet 605™.
Brilliant Violet 650™ anti-mouse CD4
C57BL/6 mouse splenocytes were stained with CD3 PE and CD4 (clone RM4-5) Brilliant Violet 650™.
