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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 570™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry3,4,5 of acetone-fixed frozen sections, and blocking of T cell activation1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 300516).
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ) 2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ) 3. Deng MC, et al. 1995. Circulation 91:1647. (IHC) 4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC) 5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) 6. Lan RY, et al. 2006. Hepatology 43:729. 7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed 8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Other Names:
T4
Structure:
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution:
T cell subset, majority of thymocytes, monocytes/macrophages
Function:
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand Receptor:
MHC class II molecules, HIV gp120, IL-16
Antigen References:
1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE
Biotin anti-human CD4
Human peripheral blood lymphocytes stained with biotinylated RPA-T4, followed by Sav-PE
FITC anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 FITC
LEAF™ Purified anti-human CD4
Human peripheral blood lymphocytes stained with LEAF™ purified RPA-T4, followed by anti-mouse IgGs FITC
PE anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE
PE/Cy5 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy5
PE/Cy7 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy7
Purified anti-human CD4
Human peripheral blood lymphocytes stained with purified RPA-T4, followed by anti-mouse IgGs FITC
APC/Cy7 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 APC/Cy7
Alexa Fluor® 488 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa Flour® 647
Pacific Blue™ anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Pacific Blue™
Brilliant Violet 421™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Alexa Fluor® 700 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa Fluor® 700
PerCP anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PerCP
PerCP/Cy5.5 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PerCP/Cy5.5
Brilliant Violet 570™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
