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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 570™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.
CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Other Names:
S152, T14, TNFRSF7
Structure:
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution:
Medullary thymocytes, T and B cell subsets, NK cells
Function:
T cell costimulation
Ligand Receptor:
CD70
Antigen References:
1. Hintzen R, et al. 1994. Immunol. Today 15:307. 2. Agematsu K, et al. 1995. J. Immunol. 154:3627.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD27
Human peripheral blood lymphocytes stained with O323 APC
Biotin anti-human CD27
Human peripheral blood lymphocytes stained with biotinylated O323, followed by Sav-PE
FITC anti-human CD27
Human peripheral blood lymphocytes stained
with O323 FITC
PE anti-human CD27
Human peripheral blood lymphocytes stained with O323 PE
Purified anti-human CD27
Human peripheral blood lymphocytes stained with purified O323, followed by anti-mouse IgGs FITC
Alexa Fluor® 647 anti-human CD27
Human peripheral blood lymphocytes stained with O323 Alexa Fluor® 647
Alexa Fluor® 700 anti-human CD27
Human peripheral blood lymphocytes stained with O323 Alexa Fluor® 700
APC/Cy7 anti-human CD27
Human peripheral blood lymphocytes stained with O323 APC/Cy7 and overlayed with isotype control
PerCP anti-human CD27
Human peripheral blood lymphocytes stained with O323 PerCP
PerCP/Cy5.5 anti-human CD27
Human peripheral blood lymphocytes stained with O323 PerCP/Cy5.5
Pacific Blue™ anti-human CD27
Human peripheral blood lymphocytes stained with O323 Pacific Blue™
Brilliant Violet 421™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 421™ (filled histogram) or mouse IgG1 Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
Brilliant Violet 650™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 650™.
Brilliant Violet 605™ anti-human CD27
Human peripheral blood lymphocytes were stained with CD27 (clone O323) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
